STAT5a overexpression 293T lysate (whole cell) (ab94124)

Overview

  • Product name
    STAT5a overexpression 293T lysate (whole cell)
  • General notes

    ab94124 is a 293T cell transfected lysate in which Human STAT5a has been transiently over-expressed using a pCMV-STAT5a plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes

  • Tested applications
    Suitable for: WBmore details

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • Purity
    Whole Cell Lysate
  • Research areas
  • Background
    Function: Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. PTM: Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Similarity: Belongs to the transcription factor STAT family. Contains 1 SH2 domain.

Applications

Our Abpromise guarantee covers the use of ab94124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

Images

  • ab94124 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-STAT5a antibody (ab57823) at 1/500 dilution

    Lane 1 : STAT5a overexpression 293T lysate (whole cell) (ab94124)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References

ab94124 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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