Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095)
Key features and details
- Mouse monoclonal [6.F1] to STAT5a (phospho Y694)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-STAT5a (phospho Y694) antibody [6.F1]
See all STAT5a primary antibodies -
Description
Mouse monoclonal [6.F1] to STAT5a (phospho Y694) -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse STAT5a (phospho Y694).
Database link: P42230 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
NK92 cell lysate; Human breast tissue; 3T3 cells. -
Clonality
Monoclonal -
Clone number
6.F1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000. Predicted molecular weight: 91 kDa.
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IHC-P | (1) |
Use a concentration of 20 - 40 µg/ml.
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ICC/IF |
1/50 - 1/1000.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 91 kDa. |
IHC-P
Use a concentration of 20 - 40 µg/ml. |
ICC/IF
1/50 - 1/1000. |
Target
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Function
Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 6776 Human
- Entrez Gene: 20850 Mouse
- Entrez Gene: 24918 Rat
- Omim: 601511 Human
- SwissProt: P42229 Human
- SwissProt: P42230 Mouse
- SwissProt: Q62771 Rat
- Unigene: 437058 Human
see all -
Alternative names
- Mammary gland factor antibody
- MGF antibody
- Signal transducer and activator of transcription 5A antibody
see all
Images
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All lanes : Anti-STAT5a (phospho Y694) antibody [6.F1] (ab106095) at 1/1000 dilution (overnight at 4°C)
Lane 1 : NK92 cell lysate, untreated
Lane 2 : NK92 cell lysate, treated with 1Ku of IL2 (30min)
Secondary
All lanes : Gt-a-Mouse IgG DyLight™800 conjugated at 1/20000 dilution
Predicted band size: 91 kDa -
ab106095 at 20µg/ml staining STAT5a in Formalin-fixed, Paraffin-embedded Human breast tissue by Immunohistochemistry.
The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. -
ab106095 staining STAT5a in 3T3 cells by Immunofluorescence (red). Green staining represents tubulin.
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab106095 has been referenced in 2 publications.
- Wang S et al. Canonical and noncanonical regulatory roles for JAK2 in the pathogenesis of rheumatoid arthritis-associated interstitial lung disease and idiopathic pulmonary fibrosis. FASEB J 36:e22336 (2022). PubMed: 35522243
- Datta P et al. Modulation of heparan sulfate biosynthesis by sodium butyrate in recombinant CHO cells. Cytotechnology 67:223-35 (2015). PubMed: 24468831