Anti-STAT5a (phospho Y694) antibody (ab30648)

Overview

• Product name
  Anti-STAT5a (phospho Y694) antibody
  See all STAT5a primary antibodies
• Description
  Rabbit polyclonal to STAT5a (phospho Y694)
• Specificity
  ab30648 detects endogenous levels of STAT5A only when phosphorylated at Tyrosine 694. Antibody ab31377 is raised against the same peptide sequence but non-phosphorylated at tyrosine 694.
• Tested applications
  Suitable for: WB, ELISA, IHC-Pmore details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic phosphopeptide derived from human STAT5a around the phosphorylation site of Tyrosine 694.

• Positive control
  • Breast carcinoma tissue; HeLa cells treated with EGF.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage buffer
  Preservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg⁺⁺ and Ca⁺⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• Purity
  Immunogen affinity purified
• Purification notes
  The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site
• Clonality
  Polyclonal
• Isotype
  IgG
• Research areas
  • Signal Transduction
  • Signaling Pathway
  • Nuclear Signaling
  • STATs

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Associated products

• Compatible Secondaries
  • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
• Corresponding non-phospho antibody
  • Anti-STAT5a antibody (ab31377)

Applications

Target

• Function
  Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.
• Sequence similarities
  Belongs to the transcription factor STAT family.
  Contains 1 SH2 domain.
• Post-translational
  modifications
  Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity.
• Cellular localization
  Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.

• Target information above from: UniProt accession P42229 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 6776 Human
  • Entrez Gene: 20850 Mouse
  • Entrez Gene: 24918 Rat
  • Omim: 601511 Human
  • SwissProt: P42229 Human
  • SwissProt: P42230 Mouse
  • SwissProt: Q62771 Rat
  • Unigene: 437058 Human

  • Unigene: 277403 Mouse
  • Unigene: 154399 Rat

  see all
• Alternative names
  • Mammary gland factor antibody
  • MGF antibody
  • Signal transducer and activator of transcription 5A antibody

  • STA5A_HUMAN antibody
  • STAT 5 antibody
  • STAT 5A antibody
  • STAT5 antibody
  • STAT5A antibody

  see all

Protocols

• Western blot protocols
• Immunohistochemistry protocols