Overview

  • Product nameAnti-STAT5b antibody
    See all STAT5b primary antibodies
  • Description
    Rabbit polyclonal to STAT5b
  • Specificityab52162 detects endogenous levels of total STAT5b protein.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic non-phosphopeptide derived from human STAT5b around the phosphorylation site of serine 731(A-P-S-P-A).

  • Positive control
    • extracts from RAW264.7 cells, treated with EGF

Properties

Applications

Our Abpromise guarantee covers the use of ab52162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
ELISA 1/40000.

Target

  • FunctionCarries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
  • Involvement in diseaseGrowth hormone insensitivity with immunodeficiency
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Signal transducer and activator of transcription 5B antibody
    • STA5B_HUMAN antibody
    • STAT5 antibody
    • Stat5b antibody
    • Transcription factor STAT5B antibody
    see all

Anti-STAT5b antibody images

  • All lanes : Anti-STAT5b antibody (ab52162) at 1/500 dilution

    Lane 1 : extracts from RAW264.7 cells, treated with EGF (200ng/ml 30min), without immunising peptide
    Lane 2 : extracts from RAW264.7 cells, treated with EGF (200ng/ml 30min), with immunising peptide


    Predicted band size : 90 kDa
    Observed band size : 90 kDa

References for Anti-STAT5b antibody (ab52162)

ab52162 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"