The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2 µg/mg of lysate.
FunctionCarries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
Involvement in diseaseGrowth hormone insensitivity with immunodeficiency
Sequence similaritiesBelongs to the transcription factor STAT family. Contains 1 SH2 domain.
Post-translational modificationsTyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway.
Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
Signal transducer and activator of transcription 5B antibody
Transcription factor STAT5B antibody
Anti-STAT5b antibody images
Immunoprecipitation - STAT5b antibody (ab65743)
Immunoprecipitation of ab65743 (2µg/mg lysate) with rat liver lysate using protein G beads (lane
1), and controls using beads alone (lane 2).
Western blot analysis by another commercially available STAT5b antibody.
References for Anti-STAT5b antibody (ab65743)
This product has been referenced in:
Elliott S et al. Lack of expression and function of erythropoietin receptors in the kidney. Nephrol Dial Transplant : (2011).
Read more (PubMed: 22167585) »