Overview

  • Product nameAnti-STAT5b antibody
    See all STAT5b primary antibodies
  • Description
    Mouse monoclonal to STAT5b
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    GST-tagged recombinant full length Human protein (391aa, according to AAH20868)

  • Positive control
    • ICC/IF:HeLa cells; WB: HeLa cell nuclear cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab76944 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 90 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionCarries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
  • Involvement in diseaseGrowth hormone insensitivity with immunodeficiency
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Signal transducer and activator of transcription 5B antibody
    • STA5B_HUMAN antibody
    • STAT5 antibody
    • Stat5b antibody
    • Transcription factor STAT5B antibody
    see all

Anti-STAT5b antibody images

  • Immunofluorescence analysis of HeLa cells using ab76944 at 10µg/ml.
  • Anti-STAT5b antibody (ab76944) at 1/500 dilution + immunogwen (GST-tagged 391aa recombinant full length human protein AAH20868) at 0.2 µg

    Secondary
    Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/5000 dilution

    Predicted band size : 90 kDa
    Observed band size : 69 kDa (why is the actual band size different from the predicted?)
  • Anti-STAT5b antibody (ab76944) at 1/500 dilution + HeLa nuclear lysate at 25 µg

    Secondary
    Goat anti-Mouse IgG (H&L) HRP-conjugated at 1/2500 dilution

    Predicted band size : 90 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • ab76944 at 10 ug/ml staining STAT5b in human Hella cells by Immunocytochemistry/ Immunofluorescence.

References for Anti-STAT5b antibody (ab76944)

ab76944 has not yet been referenced specifically in any publications.

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