Overview

  • Product name
  • Description
    Rabbit polyclonal to STAT5b
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within the N terminal amino acids 179-228 (LRIQAQFGPLAQLSPQERLSRETALQQKQVSLEAWLQREAQTLQQYRVE L) of Human STAT5b (NP_036580).

  • Positive control
    • Transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription.
  • Involvement in disease
    Growth hormone insensitivity with immunodeficiency
  • Sequence similarities
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway.
  • Cellular localization
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • Signal transducer and activator of transcription 5B antibody
    • STA5B_HUMAN antibody
    • STAT5 antibody
    • Stat5b antibody
    • Transcription factor STAT5B antibody
    see all

Images

  • Anti-STAT5b antibody (ab86273) at 1 µg/ml (5% skim milk/ PBS buffer) + transfected 293T cell lysate at 10 µg with gel concentration: 12%

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 90 kDa
    Observed band size : 90 kDa
  • ab86273 staining STAT5b in placenta tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab86273 staining STAT5b in prostate tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • ab86273 staining STAT5b in testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References

ab86273 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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