Anti-STAT6 antibody (ab44718)

Overview

  • Product nameAnti-STAT6 antibody
    See all STAT6 primary antibodies
  • Description
    Rabbit polyclonal to STAT6
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, ICC, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human STAT6 (C terminal).

  • Positive control
    • Jurkat cells. Tonsil or Placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab44718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. See Abreview.
IHC-Fr Use at an assay dependent concentration. See Abreview.
ICC Use at an assay dependent concentration. See Abreview.
WB 1/200. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).
IP 1/400.
IHC-P 1/400.

Target

  • FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12S1644 antibody
    • D12S1644 antibody
    • IL 4 STAT antibody
    • IL-4 Stat antibody
    • IL4 STAT antibody
    • Interleukin 4 Induced antibody
    • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
    • Signal transducer and activator of transcription 6 antibody
    • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
    • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
    • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
    • STAT 6 antibody
    • STAT interleukin4 induced antibody
    • STAT, interleukin4 induced antibody
    • Stat6 antibody
    • STAT6_HUMAN antibody
    • STAT6B antibody
    • STAT6C antibody
    • Transcription factor IL 4 STAT antibody
    see all

Anti-STAT6 antibody images

  • ab44718 staining STAT6 in human AGS gastric carcinoma cells by Immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilised in 0.025% Triton X in TBS and then blocked using 5% serum for 1 hour at 23°C. Samples were then incubated with primary antibody at 1/200 for 1 hour at 23°C. The secondary antibody used was a goat anti-rabbit IgG conjugated to Alexa Fluor® 568 (green) used at a 1/500 dilution.

    See Abreview

  • ab44718 staining STAT6 in human stomach tissue sections by Immunohistochemistry (frozen sections). Tissue was fixed with acetone and then blocked with 5% serum for 1 hour at 23°C followed by incubation with the primary antibody at a 1/200 dilution for 1 hour at 23°C. An undiluted HRP-conjugated goat polyclonal was used as secondary antibody.

    See Abreview

References for Anti-STAT6 antibody (ab44718)

ab44718 has not yet been referenced specifically in any publications.

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Thank you for your enquiry.

Any references we know of which have used ab44718 will be noted under the 'Specific references' tab on our online datasheet for this product. At this time we do not know of any publications using ab44718.

Read More

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH3T3 Mouse Fibroblasts)
Specification NIH3T3 Mouse Fibroblasts
Fixative Formaldehyde
Permeabilization Yes - 0.025% Triton-X in TBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Jan 12 2011

Application Immunohistochemistry free floating
Sample Mouse Tissue sections (Mouse brain sections)
Specification Mouse brain sections
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Submitted Jan 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (NIH3T3 Mouse Fibroblasts)
Total protein in input 200 µg
Specification NIH3T3 Mouse Fibroblasts
Immuno-precipitation step Protein A/G
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Submitted Jan 11 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (NIH 3T3 Mouse Fibroblasts)
Loading amount 10 µg
Specification NIH 3T3 Mouse Fibroblasts
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Dec 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Total protein in input 200 µg
Specification AGS Gastric Carcinoma cells
Immuno-precipitation step Protein A/G
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Submitted May 24 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (AGS Gastric Carcinoma cells)
Loading amount 20 µg
Specification AGS Gastric Carcinoma cells
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Apr 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (AGS Gastric Carcinoma cells)
Specification AGS Gastric Carcinoma cells
Fixative Formaldehyde
Permeabilization Yes - 0.025% Triton-X in TBS
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Apr 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Stomach)
Specification Stomach
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM citrate pH6.0
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Apr 29 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Stomach)
Specification Stomach
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Submitted Apr 29 2010

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"