Validated using a knockout cell line

Anti-STAT6 antibody [E265] (ab32108)


  • Product name
    Anti-STAT6 antibody [E265]
    See all STAT6 primary antibodies
  • Description
    Rabbit monoclonal [E265] to STAT6
  • Host species
  • Specificity
    ab32108 recognises Stat-6. It does not cross react with other STAT family members
  • Tested applications
    Suitable for: WB, Flow Cyt, IP, ICC/IFmore details
    Unsuitable for: IHC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in C-terminus of human Stat-6.

  • Positive control
    • HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab32108 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

IP 1/30.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for IHC.
  • Target

    • Function
      Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
    • Sequence similarities
      Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
    • Post-translational
      Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
    • Cellular localization
      Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
    • Information by UniProt
    • Database links
    • Alternative names
      • 12S1644 antibody
      • D12S1644 antibody
      • IL 4 STAT antibody
      • IL-4 Stat antibody
      • IL4 STAT antibody
      • Interleukin 4 Induced antibody
      • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
      • Signal transducer and activator of transcription 6 antibody
      • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
      • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
      • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
      • STAT 6 antibody
      • STAT interleukin4 induced antibody
      • STAT, interleukin4 induced antibody
      • Stat6 antibody
      • STAT6_HUMAN antibody
      • STAT6B antibody
      • STAT6C antibody
      • Transcription factor IL 4 STAT antibody
      see all


    • Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: STAT6 knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: Raji whole cell lysate (20 µg)

      Lanes 1 - 4: Merged signal (red and green). Green - ab32108 observed at 94 kDa. Red - loading control, ab9484, observed at 37 kDa.

      ab32108 was shown to specifically react with STAT6 in wild-type HAP1 cells as signal was lost in STAT6 knockout cells. Wild-type and STAT6 knockout samples were subjected to SDS-PAGE. Ab32108 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/5000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunofluorescent staining of HeLa cells using ab32108
    • Anti-STAT6 antibody [E265] (ab32108) at 1/5000 dilution
      Predicted band size: 94 kDa
      Observed band size: 100 kDa (why is the actual band size different from the predicted?)


    ab32108 has not yet been referenced specifically in any publications.

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