Overview

  • Product nameAnti-STAT6 antibody [YE361]
    See all STAT6 primary antibodies
  • Description
    Rabbit monoclonal [YE361] to STAT6
  • SpecificityThe antibody does not cross-react with other Stat family members.
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human STAT6 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P42226

  • Positive control
    • NIH 3T3 cell lysate, human skin carcinoma tissue and HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Alternative versions available:
    Anti-STAT6 antibody (Alexa Fluor® 488) [YE361] (ab196478)

    Anti-STAT6 antibody (Alexa Fluor® 647) [YE361] (ab196928)
    Anti-STAT6 antibody (HRP) [YE361] (ab196480)

    Anti-STAT6 antibody (Alexa Fluor® 594) [YE361] (ab207012)

    Anti-STAT6 antibody (Alexa Fluor® 555) [YE361] (ab207014)

    Anti-STAT6 antibody (Alexa Fluor® 568) [YE361] (ab207015)

Properties

Applications

Our Abpromise guarantee covers the use of ab32520 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 94 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

ICC/IF 1/100.
IP 1/20 - 1/80.
Flow Cyt Use at an assay dependent concentration.

Target

  • FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12S1644 antibody
    • D12S1644 antibody
    • IL 4 STAT antibody
    • IL-4 Stat antibody
    • IL4 STAT antibody
    • Interleukin 4 Induced antibody
    • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
    • Signal transducer and activator of transcription 6 antibody
    • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
    • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
    • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
    • STAT 6 antibody
    • STAT interleukin4 induced antibody
    • STAT, interleukin4 induced antibody
    • Stat6 antibody
    • STAT6_HUMAN antibody
    • STAT6B antibody
    • STAT6C antibody
    • Transcription factor IL 4 STAT antibody
    see all

Anti-STAT6 antibody [YE361] images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

  • Immunofluorescence staining of HeLa cells with purified ab32520 at a working dilution of 1/100, counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab32520 was used at a dilution of 1/500 followed by an Alexa Fluor® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.

  • All lanes : Anti-STAT6 antibody [YE361] (ab32520) at 1/1000 dilution (purified)

    Lane 1 : NIH/3T3 cell lysate
    Lane 2 : Raw 264.7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 94 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Flow Cytometry analysis of HeLa(human cervix adenocarcinoma) cells labeling STAT6 with purified ab32520 at 1/30 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Anti-STAT6 antibody [YE361] (ab32520) at 1/20000 dilution (purified) + Raji cell lysate at 10 µg

    Secondary
    HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

    Predicted band size : 94 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)

    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of paraffin embedded human kidney with purified ab32520 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
  • ab32520 (purified) at 1/20 immunoprecipitating STAT6 in 10 μg NIH/3T3 (Lanes 1 and 2, observed at 100 kDa). Lane 3 - PBS. For western blotting, HRP Veriblot for IP (ab131366) was used as the secondary antibody (1/10 000). Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
  • Anti-STAT6 antibody [YE361] (ab32520) at 1/1000 dilution (unpurified) + NIH 3T3 cell lysate

    Predicted band size : 94 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human transitional cell carcinoma of bladder tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human solitary fibrous tumor tissue sections labelling STAT6 with unpurified ab32520 at a dilution of 1/1000. HRP goat anti-rabbit (ab97051) was used at a dilution of 1/500. The antigen retrieval solution was Tris-EDTA buffer, pH 9.0.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling STAT6 with unpurified ab32520 at 1/100.

  • Immunocytochemistry/Immunofluorescence analyis of HeLa cells labelling STAT6 with unpurified ab32520 at 1/100.

References for Anti-STAT6 antibody [YE361] (ab32520)

This product has been referenced in:
  • Bai Y  et al. Pdcd4 Is Involved in the Formation of Stress Granule in Response to Oxidized Low-Density Lipoprotein or High-Fat Diet. PLoS One 11:e0159568 (2016). WB ; Mouse . Read more (PubMed: 27454120) »
  • Cheah AL  et al. STAT6 rabbit monoclonal antibody is a robust diagnostic tool for the distinction of solitary fibrous tumour from its mimics. Pathology 46:389-95 (2014). IHC-P ; Human . Read more (PubMed: 24977739) »

See all 2 Publications for this product

Product Wall

Application Western blot
Sample Mouse Tissue lysate - whole (LUNG)
Gel Running Conditions Reduced Denaturing (7.5)
Loading amount 30 µg
Treatment Oxidant 24hr
Specification LUNG
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted Dec 11 2015

Thank you for contacting us.



The antibody is from tissue culture supernatant.



The Jurkat lysate will serve as a positive control for STAT6, in addition to IRF7. Although ab32520 has not been tested on Jurkat, other STAT6 antibodies have been, and the results demonstrate that Jurkat expresses this protein. For an example western ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Thank you for taking the time to complete our questionnaire and contact us. The details you have kindly provided will provide us with vital information for our monitoring of product quality.

I appreciate the time you have spent in the labora...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that we monitor feedback closely on a weekly basis and we are not current...

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Thank you for contacting Abcam regarding ab32520.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the st...

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Thank you for contacting us regarding ab32520. I'm sorry to hear you are experiencing problems with this antibody which has been tested in IHC-P and should work in lung tissue of mouse and human. Could you please confirm the species of your samples? If...

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Thank you for your reply. For ab32520, HIER using Citrate buffer pH 6 was used as the pretreatment on FFPE tissue. You may find a protocol for this at the following link: http://www.abcam.com/assets/pdf/protocols/IHC-paraffin%20protocol%20(IHC...

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