Anti-STAT6 (phospho Y641) antibody (ab28829)

Overview

  • Product nameAnti-STAT6 (phospho Y641) antibody
    See all STAT6 primary antibodies
  • Description
    Rabbit polyclonal to STAT6 (phospho Y641)
  • Specificityab28829 recognises Phospho-STAT6(Tyr641).
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized phosphopeptide derived from human STAT6 around the phosphorylation site of Tyrosine 641.

  • Positive control
    • Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab28829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 94 kDa.

Target

  • FunctionCarries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
  • Sequence similaritiesBelongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
  • Cellular localizationCytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12S1644 antibody
    • D12S1644 antibody
    • IL 4 STAT antibody
    • IL-4 Stat antibody
    • IL4 STAT antibody
    • Interleukin 4 Induced antibody
    • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
    • Signal transducer and activator of transcription 6 antibody
    • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
    • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
    • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
    • STAT 6 antibody
    • STAT interleukin4 induced antibody
    • STAT, interleukin4 induced antibody
    • Stat6 antibody
    • STAT6_HUMAN antibody
    • STAT6B antibody
    • STAT6C antibody
    • Transcription factor IL 4 STAT antibody
    see all

Anti-STAT6 (phospho Y641) antibody images



  • Predicted band size : 94 kDa

    Western blot analysis of lysates from HeLa cells treated with IL-4, using STAT6 (Phospho-Tyr641) Antibody. The lane on the right is blocked with the phospho peptide.

     

     

     

  • Immunohistochemistry (Formalin-fixed paraffin-embedded sections) of breast carcinom using ab28829 preincubated with synthesised phospho-peptide.
  • ab28829 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody.

    See Abreview

References for Anti-STAT6 (phospho Y641) antibody (ab28829)

ab28829 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Control and IL-13 Treated Mouse Lungs)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH6, Citrate based
Permeabilization No
Specification Control and IL-13 Treated Mouse Lungs
Blocking step Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative 10% NBF
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Ms. Premier Laboratory

Verified customer

Submitted Apr 05 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step None
Sample Human Tissue sections (Aorta)
Specification Aorta
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample Human Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: Proteinase K
Sample Human Tissue sections (Aorta)
Specification Aorta
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 24 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rhesus monkey Tissue sections (Colon)
Specification Colon
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Permeabilization Yes - 0.1% Triton-X-100
Blocking step Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2013

Thank you very much for having provided your feedback in the last survey on the resolution of the complaints. We completely understand your concern and apologize for the inconvenience caused by the faulty vial. This is obviously not what we expect neit...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Lung)
Specification Lung
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
Username

Abcam user community

Verified customer

Submitted Apr 27 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"