Anti-STAT6 (phospho Y641) antibody (ab28829)

Overview

  • Product name
    Anti-STAT6 (phospho Y641) antibody
    See all STAT6 primary antibodies
  • Description
    Rabbit polyclonal to STAT6 (phospho Y641)
  • Specificity
    ab28829 recognises Phospho-STAT6(Tyr641).
  • Tested applications
    Suitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthesized phosphopeptide derived from human STAT6 around the phosphorylation site of Tyrosine 641.

  • Positive control
    • Breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab28829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ELISA 1/10000.
WB 1/500 - 1/1000. Predicted molecular weight: 94 kDa.

Target

  • Function
    Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling.
  • Sequence similarities
    Belongs to the transcription factor STAT family.
    Contains 1 SH2 domain.
  • Post-translational
    modifications
    Tyrosine phosphorylated following stimulation by IL-4 and IL-3.
  • Cellular localization
    Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation.
  • Information by UniProt
  • Database links
  • Alternative names
    • 12S1644 antibody
    • D12S1644 antibody
    • IL 4 STAT antibody
    • IL-4 Stat antibody
    • IL4 STAT antibody
    • Interleukin 4 Induced antibody
    • Interleukin 4 Induced Transcription Factor IL4 STAT antibody
    • Signal transducer and activator of transcription 6 antibody
    • Signal Transducer And Activator Of Transcription 6 Interleukin 4 Induced antibody
    • Signal Transducer And Activator Of Transcription 6 Nirs Variant 1 antibody
    • Signal transducer and activator of transcription 6, interleukin 4 induced antibody
    • STAT 6 antibody
    • STAT interleukin4 induced antibody
    • STAT, interleukin4 induced antibody
    • Stat6 antibody
    • STAT6_HUMAN antibody
    • STAT6B antibody
    • STAT6C antibody
    • Transcription factor IL 4 STAT antibody
    see all

Anti-STAT6 (phospho Y641) antibody images



  • Predicted band size : 94 kDa

    Western blot analysis of lysates from HeLa cells treated with IL-4, using STAT6 (Phospho-Tyr641) Antibody. The lane on the right is blocked with the phospho peptide.

     

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling STAT6 (phospho Y641) with ab28829. Right pane - blocked with the phospho peptide.

  • ab28829 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody.

    See Abreview

References for Anti-STAT6 (phospho Y641) antibody (ab28829)

This product has been referenced in:
  • Dong J & Ma Q In vivo activation of a T helper 2-driven innate immune response in lung fibrosis induced by multi-walled carbon nanotubes. Arch Toxicol 90:2231-48 (2016). IHC ; Mouse . Read more (PubMed: 27106021) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (lung)
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 19 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted May 19 2017

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Control and IL-13 Treated Mouse Lungs)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6, Citrate based
Permeabilization
No
Specification
Control and IL-13 Treated Mouse Lungs
Blocking step
Serum Free Protein Block (Dako) as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C
Fixative
10% NBF
Username

Premier Laboratory

Verified customer

Submitted Apr 05 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
None
Sample
Human Tissue sections (Aorta)
Specification
Aorta
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Sample
Human Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
None
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase K
Sample
Human Tissue sections (Aorta)
Specification
Aorta
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 24 2013

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Colon)
Specification
Colon
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
Yes - 0.1% Triton-X-100
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 08 2013

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