Anti-STAT6 (phospho Y641) antibody (ab28829)
Key features and details
- Rabbit polyclonal to STAT6 (phospho Y641)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-STAT6 (phospho Y641) antibody
See all STAT6 primary antibodies -
Description
Rabbit polyclonal to STAT6 (phospho Y641) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human STAT6 (phospho Y641). Synthesized phosphopeptide derived from human STAT6 around the phosphorylation site of Tyrosine 641.
Database link: P42226 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (6) |
1/50 - 1/100.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 94 kDa.
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Notes |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 94 kDa. |
Target
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Function
Carries out a dual function: signal transduction and activation of transcription. Involved in interleukin-4 signalling. -
Sequence similarities
Belongs to the transcription factor STAT family.
Contains 1 SH2 domain. -
Post-translational
modificationsTyrosine phosphorylated following stimulation by IL-4 and IL-3. -
Cellular localization
Cytoplasm. Nucleus. Translocated into the nucleus in response to phosphorylation. - Information by UniProt
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Database links
- Entrez Gene: 6778 Human
- Omim: 601512 Human
- SwissProt: P42226 Human
- Unigene: 524518 Human
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Alternative names
- 12S1644 antibody
- D12S1644 antibody
- IL 4 STAT antibody
see all
Images
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Lanes 1-2 : STAT6 antibody
Lanes 3-4 : Anti-STAT6 (phospho Y641) antibody (ab28829) at 1/1000 dilution
Lanes 1 & 3 : K562 cells treated with 100 ng/mL IL4
Lanes 2 & 4 : K562 untreated cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Dylight 800 secondary antibody at 1/10000 dilution
Predicted band size: 94 kDaLoading control is Actin
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Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-STAT6 (phospho Y641) antibody (ab28829) at 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling STAT6 (phospho Y641) with ab28829. Right pane - blocked with the phospho peptide.
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ab28829 at 1/100 staining mouse lung tissue sections by IHC-P. The tissue was formaldehyde fixed and blocked with serum. A heat mediated antigen retrieval step was performed prior to incubation with the antibody. Ab6720 (Goat polyclonal to Rabbit IgG H&L (biotin)) was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (34)
ab28829 has been referenced in 34 publications.
- Yu JE et al. Anti-Chi3L1 antibody suppresses lung tumor growth and metastasis through inhibition of M2 polarization. Mol Oncol 16:2214-2234 (2022). PubMed: 34861103
- Hara T et al. CD73+ Epithelial Progenitor Cells That Contribute to Homeostasis and Renewal Are Depleted in Eosinophilic Esophagitis. Cell Mol Gastroenterol Hepatol 13:1449-1467 (2022). PubMed: 35108658
- Lin YT et al. JAK2 Phosphorylation Signals and Their Associated Cytokines Involved in Chronic Rhinosinusitis with Nasal Polyps and Correlated with Disease Severity. Biomolecules 11:N/A (2021). PubMed: 34356683
- Zhang C et al. Interleukins 4 and 13 drive lipid abnormalities in skin cells through regulation of sex steroid hormone synthesis. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 34521750
- Huang TC et al. Thrombomodulin facilitates peripheral nerve regeneration through regulating M1/M2 switching. J Neuroinflammation 17:240 (2020). PubMed: 32799887