Overview

  • Product nameAnti-Statherin antibody
  • Description
    Rabbit polyclonal to Statherin
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Saccharomyces cerevisiae
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 1-50 (MKFLVFAFIL ALMVSMIGAD SSEEKFLRRI GRFGYGYGPY QPVPEQPLYP) of Human Statherin (NP_003145).

  • Positive control
    • Human fetal thymus lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab97950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 7 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IP Use at an assay dependent concentration. See Abreview.

Target

  • FunctionSalivary protein that stabilizes saliva supersaturated with calcium salts by inhibiting the precipitation of calcium phosphate salts. It also modulates hydroxyapatite crystal formation on the tooth surface.
  • Tissue specificitySecreted by parotid and submandibular glands.
  • Sequence similaritiesBelongs to the histatin/statherin family.
  • Post-translational
    modifications
    Substrate for transglutaminase-2. More than 95% of the cyclized peptide is cyclo-statherin Q-37, and less than 5% is cyclo-statherin Q-39. Cyclized forms account for about 1% of total statherin in saliva.
    Sulfated on tyrosine residues.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • STAT_HUMAN antibody
    • STATH antibody
    • Statherin antibody
    • Statherin precursor antibody
    • STR antibody
    see all

Anti-Statherin antibody images

  • Anti-Statherin antibody (ab97950) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal thymus lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 7 kDa
  • ab97950 Immunoprecipitating Stratherin, harvested synthetic protein from Human Tissue lysate. 20µg of Tissue lysate was incubated with primary antibody (1/200 in TBS buffer) and matrix (Protein G) for 24 hours at 4°C. For western blotting ab97950 (1/1000) was used to confirm successful immunoprecipation.

    See Abreview

References for Anti-Statherin antibody (ab97950)

This product has been referenced in:
  • Silva-Boghossian CM  et al. Quantitative proteomic analysis of gingival crevicular fluid in different periodontal conditions. PLoS One 8:e75898 (2013). ELISA ; Human . Read more (PubMed: 24098404) »

See 1 Publication for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunoprecipitation
Sample Human Tissue lysate - other (Harvested synthetic protein)
Total protein in input 20 µg
Specification Harvested synthetic protein
Immuno-precipitation step Protein G
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Submitted Aug 03 2012

I am very pleased to hear you would like to accept our offer and test ab97950 in IP. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for IP and include this code in the “...

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Abcam does not currently have a recommneded protocol specifically for co-IP. I was able to find so information which may be valuable to you at a competators website (http://www.piercenet.com/browse.cfm?fld...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"