Recombinant
Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
- Datasheet
- Specific References (8)
- Protocols
Overview
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Product nameAnti-Stathmin 1 antibody [EP1573Y]
See all Stathmin 1 primary antibodies -
DescriptionRabbit monoclonal [EP1573Y] to Stathmin 1
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Tested applicationsSuitable for: ICC/IF, WB, IP, Flow Cyt, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Stathmin 1 aa 100 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P16949 -
Positive control
- PC12 cytoplasmic lysate, HeLa cells and lymph node tissue
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General notes
This product is a recombinant rabbit monoclonal antibody.
Alternative versions available:
Anti-Stathmin 1 antibody (Alexa Fluor® 488) [EP1573Y] (ab199735)
Anti-Stathmin 1 antibody (Alexa Fluor® 647) [EP1573Y] (ab199812)
Anti-Stathmin 1 antibody (HRP) [EP1573Y] (ab199466)
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
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PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEP1573Y
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Immunohistochemistry kits
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab52630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/100 - 1/250. | |
| WB | 1/50000. Detects a band of approximately 19 kDa. | |
| IP | 1/50. | |
| Flow Cyt | 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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| IHC-P | Use at an assay dependent concentration. |
Target
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FunctionInvolved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
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Tissue specificityUbiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
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Sequence similaritiesBelongs to the stathmin family.
Contains 1 SLD (stathmin-like) domain. -
Post-translational
modificationsMany different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. -
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
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Database links
- Entrez Gene: 3925 Human
- Entrez Gene: 16765 Mouse
- Entrez Gene: 29332 Rat
- Omim: 151442 Human
- SwissProt: P16949 Human
- SwissProt: P54227 Mouse
- SwissProt: P13668 Rat
- Unigene: 209983 Human
see all -
Alternative names
- C1orf215 antibody
- Lag antibody
- LAP 18 antibody
see all
Anti-Stathmin 1 antibody [EP1573Y] images
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![Western blot - Stathmin 1 antibody [EP1573Y] (ab52630)](http://a.abcam.com/ps/products/52/ab52630/Images/ab52630_WB_1.jpg)
Western blot - Stathmin 1 antibody [EP1573Y] (ab52630)Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/500000 dilution + PC12 lysate at 10 µg
Secondary
goat anti-rabbit HRP at 1/2000 dilution
Observed band size : 18 kDa (why is the actual band size different from the predicted?) -
![Immunocytochemistry/ Immunofluorescence - Stathmin 1 antibody [EP1573Y] (ab52630)](http://a.abcam.com/ps/products/52/ab52630/Images/ab52630_ICC_1.jpg)
Immunocytochemistry/ Immunofluorescence - Stathmin 1 antibody [EP1573Y] (ab52630)ab52630 stained HeLa cells -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)](http://a.abcam.com/ps/products/52/ab52630/Images/ab52630_IHC_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Stathmin 1 antibody [EP1573Y] (ab52630)ab52630, at a 1/250 dilution, staining human Stathmin 1 in lymph node tissue, using Immunohistochemistry, Paraffin embedded sections. -
](http://a.abcam.com/ps/products/52/ab52630/Images/Stathmin-1-Primary-antibodies-ab52630-9.jpg)
Flow Cytometry-Stathmin 1 antibody [EP1573Y](ab52630)Overlay histogram showing Jurkat cells stained with ab52630 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52630, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions. -
![Immunoprecipitation - Stathmin 1 antibody [EP1573Y] (ab52630)](http://a.abcam.com/ps/products/52/ab52630/Images/Stathmin-1-Primary-antibodies-ab52630-10.jpg)
Immunoprecipitation - Stathmin 1 antibody [EP1573Y] (ab52630)Image courtesy of Qifeng Lin by Abreview.Whole cell lysate prepared from human colon cells was loaded at 20µg. The immunoprecipitation step was performed using Protein A/G. ab52630 used at a 1/200 dilution for 12 hours at 4°C. For WB ab52630 used at a 1/10000 dilution.
References for Anti-Stathmin 1 antibody [EP1573Y] (ab52630)
This product has been referenced in:
- Yadav P et al. Neurofilament depletion improves microtubule dynamics via modulation of Stat3/stathmin signaling. Acta Neuropathol 132:93-110 (2016). IP ; Mouse . Read more (PubMed: 27021905) »
- Liu S et al. Tyrosine kinase LYN is an oncotarget in human cervical cancer: A quantitative proteomic based study. Oncotarget 7:75468-75481 (2016). WB ; Human . Read more (PubMed: 27690342) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"