Anti-Stathmin 1 antibody [EP1573Y] (ab52630)

Anti-Stathmin 1 antibody [EP1573Y] (ab52630) at 1/500000 dilution + PC12 lysate at 10 µg

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Observed band size: 18 kDa (why is the actual band size different from the predicted?)

ab52630 stained HeLa cells

ab52630, at a 1/250 dilution, staining human Stathmin 1 in lymph node tissue, using Immunohistochemistry, Paraffin embedded sections.

Overlay histogram showing Jurkat cells stained with ab52630 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52630, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) ( 1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

Whole cell lysate prepared from human colon cells was loaded at 20µg. The immunoprecipitation step was performed using Protein A/G. ab52630 used at a 1/200 dilution for 12 hours at 4°C. For WB ab52630 used at a 1/10000 dilution.

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