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Synthetic peptide corresponding to Human Stathmin 1. synthesized phosphopeptide derived from human Stathmin 1 around the phosphorylation site of serine 16 (R-A-SP-G-Q)
Database link: P16949
Our Abpromise guarantee covers the use of ab47328 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000.|
|ICC/IF||1/100 - 1/500.|
|IHC-P||1/50 - 1/100.|
ab47328 staining Stathmin1 (phospho S16) in Rat hippocampul neurons by Immunocytochemistry/ Immunofluorescence. Cultures of primary hippocampal neurons were prepared from embryonic day 18 rat embryos. The cells were transfected with GFP-shc (control shRNA vector) as control or GFP-sh2 Kidins220 / ARMS-specific shRNA, using serum-free medium with Lipofectamine. The cells were grown on coverslips and fixed for 5 min in 4% paraformaldehyde containing 4% sucrose in phosphate-buffered saline at 37 °C. Cells were then permeabilized with 0.2% Triton X-100 in phosphate-buffered saline during 5 min at room temperature. After blocking (5% bovine serum albumin in phosphate-buffered saline for 1 h), cells were incubated with the primary antibody. An Alexa Fluor®647 conjugated secondary antibody was used.