Anti-Stathmin 1 (phospho S24) antibody (ab47398)

Overview

  • Product nameAnti-Stathmin 1 (phospho S24) antibody
    See all Stathmin 1 primary antibodies
  • Description
    Rabbit polyclonal to Stathmin 1 (phospho S24)
  • SpecificityThis antibody is specific for Stathmin 1, only when phosphorylated at serine 24.
  • Tested applicationsSuitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized phosphopeptide derived from human Stathmin 1 around the phosphorylation site of serine 24 (I-L-SP-P-R)

  • Positive control
    • Extracts from Jurkat cells and human breast carcinoma tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 22529300
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

Target

  • FunctionInvolved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
  • Tissue specificityUbiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
  • Sequence similaritiesBelongs to the stathmin family.
    Contains 1 SLD (stathmin-like) domain.
  • Post-translational
    modifications
    Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
  • Cellular localizationCytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • C1orf215 antibody
    • Lag antibody
    • LAP 18 antibody
    • LAP18 antibody
    • Leukemia associated phosphoprotein p18 antibody
    • Leukemia-associated phosphoprotein p18 antibody
    • Metablastin antibody
    • Oncoprotein 18 antibody
    • OP 18 antibody
    • Op18 antibody
    • p18 antibody
    • p19 antibody
    • Phosphoprotein 19 antibody
    • Phosphoprotein p19 antibody
    • pp17 antibody
    • pp19 antibody
    • PR22 antibody
    • Pr22 protein antibody
    • Prosolin antibody
    • Protein Pr22 antibody
    • SMN antibody
    • Stathmin antibody
    • Stathmin1 antibody
    • STMN 1 antibody
    • Stmn1 antibody
    • STMN1_HUMAN antibody
    see all

Anti-Stathmin 1 (phospho S24) antibody images

  • All lanes : Anti-Stathmin 1 (phospho S24) antibody (ab47398) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells treated with PMA
    Lane 2 : Extracts from Jurkat cells untreated


    Predicted band size : 17 kDa
    Observed band size : 19 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab47398 at a 1/50 dilution.

    Left image : Un-treated

    Right image : Treated with P-Peptide

References for Anti-Stathmin 1 (phospho S24) antibody (ab47398)

This product has been referenced in:
  • Filbert EL  et al. Stathmin regulates microtubule dynamics and microtubule organizing center polarization in activated T cells. J Immunol 188:5421-7 (2012). WB, ICC/IF . Read more (PubMed: 22529300) »

See 1 Publication for this product

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