Anti-Stathmin 1 (phospho S24) antibody [EP2124Y] (ab62336)

Overview

  • Product name
    Anti-Stathmin 1 (phospho S24) antibody [EP2124Y]
    See all Stathmin 1 primary antibodies
  • Description
    Rabbit monoclonal [EP2124Y] to Stathmin 1 (phospho S24)
  • Specificity
    This antibody detects Stathmin 1 phosphorylated at Serine 24.
  • Tested applications
    Suitable for: WB, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Phospho specific peptide corresponding to residues surrounding Serine 24 of human Stathmin 1.

  • Positive control
    • HeLa cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab62336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 19 kDa (predicted molecular weight: 17 kDa).
ICC 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Involved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
    • Tissue specificity
      Ubiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
    • Sequence similarities
      Belongs to the stathmin family.
      Contains 1 SLD (stathmin-like) domain.
    • Post-translational
      modifications
      Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
    • Cellular localization
      Cytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • C1orf215 antibody
      • Lag antibody
      • LAP 18 antibody
      • LAP18 antibody
      • Leukemia associated phosphoprotein p18 antibody
      • Leukemia-associated phosphoprotein p18 antibody
      • Metablastin antibody
      • Oncoprotein 18 antibody
      • OP 18 antibody
      • Op18 antibody
      • p18 antibody
      • p19 antibody
      • Phosphoprotein 19 antibody
      • Phosphoprotein p19 antibody
      • pp17 antibody
      • pp19 antibody
      • PR22 antibody
      • Pr22 protein antibody
      • Prosolin antibody
      • Protein Pr22 antibody
      • SMN antibody
      • Stathmin antibody
      • Stathmin1 antibody
      • STMN 1 antibody
      • Stmn1 antibody
      • STMN1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Stathmin 1 (phospho S24) antibody [EP2124Y] (ab62336) at 1/1000 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with FBS+Calyculin A)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP conjugate at 1/2000 dilution

      Predicted band size : 17 kDa
      Observed band size : 19 kDa (why is the actual band size different from the predicted?)

    References

    This product has been referenced in:
    • Alesi GN  et al. RSK2 signals through stathmin to promote microtubule dynamics and tumor metastasis. Oncogene 35:5412-5421 (2016). Read more (PubMed: 27041561) »
    • Zhao E  et al. Stathmin mediates hepatocyte resistance to death from oxidative stress by down regulating JNK. PLoS One 9:e109750 (2014). WB . Read more (PubMed: 25285524) »

    See all 2 Publications for this product

    Customer reviews and Q&As

    Thank you for confirming this information and for your help and cooperation with this case.

    As requested, I have asked our accounting department to issue you with a credit note. This can then be redeemed against the invoice of a future order...

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    Thank you for your message and for providing this further information.

    I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time they have spent on these experiments, it is disappointing th...

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    Thank you for your message.

    To confirm:

    1. Growing cells were starved for 24hr in the medium without FBS.

    2. Growing cells treated the with 20% FBS for 3hr.

    3. Add calyculin A to the growing cells at the conc...

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    Thank you for your reply and for kindly providing this information.

    Reviewing the details, I can suggest my main concern is still that the cells have not been treated.

    Here is the treatment which was used in testing the cells (as ...

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    Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear the customer has had difficulty obtaining satisfactory results from this antibody.

    The details you have kindly provided will enable us to investig...

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