Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

Overview

  • Product nameAnti-Stathmin 1 (phospho S63) antibody [EPR1574]
    See all Stathmin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1574] to Stathmin 1 (phospho S63)
  • Specificityab76583 only detects Stathmin 1 phosphorylated on Serine 62. The antibody immunogen shares 86% homology with Stathmin-2, therefore it is possible that the antibody will cross-react with Stathmin-2 when phosphorylated at serine 97. This has not been assessed experimentally.
  • Tested applicationsSuitable for: Dot blot, IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    corresponding to Human Stathmin 1.

  • Positive control
    • HeLa cell lysates treated with Calyculin A; human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab76583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/1000.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/2500 - 1/10000. Predicted molecular weight: 17 kDa.
IP 1/20.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionInvolved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
    • Tissue specificityUbiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
    • Sequence similaritiesBelongs to the stathmin family.
      Contains 1 SLD (stathmin-like) domain.
    • Post-translational
      modifications
      Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • C1orf215 antibody
      • Lag antibody
      • LAP 18 antibody
      • LAP18 antibody
      • Leukemia associated phosphoprotein p18 antibody
      • Leukemia-associated phosphoprotein p18 antibody
      • Metablastin antibody
      • Oncoprotein 18 antibody
      • OP 18 antibody
      • Op18 antibody
      • p18 antibody
      • p19 antibody
      • Phosphoprotein 19 antibody
      • Phosphoprotein p19 antibody
      • pp17 antibody
      • pp19 antibody
      • PR22 antibody
      • Pr22 protein antibody
      • Prosolin antibody
      • Protein Pr22 antibody
      • SMN antibody
      • Stathmin antibody
      • Stathmin1 antibody
      • STMN 1 antibody
      • Stmn1 antibody
      • STMN1_HUMAN antibody
      see all

    Anti-Stathmin 1 (phospho S63) antibody [EPR1574] images

    • All lanes : Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583) at 1/10000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
    • ab76583, at a 1/250 dilution, staining Stathmin 1 in paraffin embedded human brain tissue by Immunohistochemistry.
    • Dot blot analysis of Stathmin 1 (phospho S63) phospho peptide (Lane 1) and Stathmin 1 non-phospho peptide (Lane 2) labeling Stathmin 1 (phospho S63) with ab76583 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG) (H+L) at 1/100 000 was used as the secondary antibody.

      Blocking and diluting buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.



    • Predicted band size : 17 kDa

      Image courtesy of Qifeng Lin by Abreview.

      Alkaline phosphatase treatment removes S63 phosphorylation. Samples treated with phosphatase were run alongside the normal lysate, and the phosph-S63 signal is not detected after phosphatase treatment, thus suggesting the signal is very specific to the phosphorylated S63.
      Whole cell lysate prepared from a human colon cancer cell line was loaded at 20µg.
      ab76583 used at a 1/1000 dilution.
      Secondary used was an HRP conjugated goat anti-rabbit polyclonal used at a 1/10000 dilution.

      See Abreview

    References for Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    This product has been referenced in:
    • Yip YY  et al. cAMP-dependent protein kinase and c-Jun N-terminal kinase mediate stathmin phosphorylation for the maintenance of interphase microtubules during osmotic stress. J Biol Chem 289:2157-69 (2014). Read more (PubMed: 24302736) »
    • Tian X  et al. Microtubule dynamics control HGF-induced lung endothelial barrier enhancement. PLoS One 9:e105912 (2014). WB ; Human . Read more (PubMed: 25198505) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (colon carcinoma cell line)
    Loading amount 20 µg
    Specification colon carcinoma cell line
    Treatment alkaline phosphatase treatment as negative control
    Gel Running Conditions Reduced Denaturing (12.5%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Qifeng LIN

    Verified customer

    Submitted Dec 27 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"