Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)


  • Product nameAnti-Stathmin 1 (phospho S63) antibody [EPR1574]
    See all Stathmin 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR1574] to Stathmin 1 (phospho S63)
  • Specificityab76583 only detects Stathmin 1 phosphorylated on Serine 62.
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding Serine 62 of human Stathmin 1

  • Positive control
    • HeLa cell lysates treated with Calyculin A; human brain tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated "PUR" on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.



Our Abpromise guarantee covers the use of ab76583 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-P: 1/250 - 1/500. Antigen retrieval is recommended before commencing with IHC staining protocol.
    IP: 1/20.
    WB: 1/2500 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

    Is unsuitable for Flow Cyt or ICC.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionInvolved in the regulation of the microtubule (MT) filament system by destabilizing microtubules. Prevents assembly and promotes disassembly of microtubules. Phosphorylation at Ser-16 may be required for axon formation during neurogenesis. Involved in the control of the learned and innate fear.
    • Tissue specificityUbiquitous. Expression is strongest in fetal and adult brain, spinal cord, and cerebellum, followed by thymus, bone marrow, testis, and fetal liver. Expression is intermediate in colon, ovary, placenta, uterus, and trachea, and is readily detected at substantially lower levels in all other tissues examined. Lowest expression is found in adult liver. Present in much greater abundance in cells from patients with acute leukemia of different subtypes than in normal peripheral blood lymphocytes, non-leukemic proliferating lymphoid cells, bone marrow cells, or cells from patients with chronic lymphoid or myeloid leukemia.
    • Sequence similaritiesBelongs to the stathmin family.
      Contains 1 SLD (stathmin-like) domain.
    • Post-translational
      Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity.
    • Cellular localizationCytoplasm > cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • C1orf215 antibody
      • Lag antibody
      • LAP 18 antibody
      • LAP18 antibody
      • Leukemia associated phosphoprotein p18 antibody
      • Leukemia-associated phosphoprotein p18 antibody
      • Metablastin antibody
      • Oncoprotein 18 antibody
      • OP 18 antibody
      • Op18 antibody
      • p18 antibody
      • p19 antibody
      • Phosphoprotein 19 antibody
      • Phosphoprotein p19 antibody
      • pp17 antibody
      • pp19 antibody
      • PR22 antibody
      • Pr22 protein antibody
      • Prosolin antibody
      • Protein Pr22 antibody
      • SMN antibody
      • Stathmin antibody
      • Stathmin1 antibody
      • STMN 1 antibody
      • Stmn1 antibody
      • STMN1_HUMAN antibody
      see all

    Anti-Stathmin 1 (phospho S63) antibody [EPR1574] images

    • ab76583, at a 1/250 dilution, staining Stathmin 1 in paraffin embedded human brain tissue by Immunohistochemistry.
    • All lanes : Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583) at 1/10000 dilution

      Lane 1 : HeLa cell lysate, untreated
      Lane 2 : HeLa cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa

    • Predicted band size : 17 kDa

      Image courtesy of Qifeng Lin by Abreview.

      See Abreview

    References for Anti-Stathmin 1 (phospho S63) antibody [EPR1574] (ab76583)

    This product has been referenced in:
    • Yip YY  et al. cAMP-dependent protein kinase and c-Jun N-terminal kinase mediate stathmin phosphorylation for the maintenance of interphase microtubules during osmotic stress. J Biol Chem 289:2157-69 (2014). Read more (PubMed: 24302736) »
    • Tian X  et al. Microtubule dynamics control HGF-induced lung endothelial barrier enhancement. PLoS One 9:e105912 (2014). WB ; Human . Read more (PubMed: 25198505) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (colon carcinoma cell line)
    Loading amount 20 µg
    Specification colon carcinoma cell line
    Treatment alkaline phosphatase treatment as negative control
    Gel Running Conditions Reduced Denaturing (12.5%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Mr. Qifeng LIN

    Verified customer

    Submitted Dec 27 2011