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Our Abpromise guarantee covers the use of ab60724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. The detection limit for recombinant tagged STAU2 is approximately 0.03ng/ml as a capture antibody.|
|WB||Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 63 kDa).|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20668554|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||Use 0.1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|