• Product nameAnti-Staufen antibody
    See all Staufen primary antibodies
  • Description
    Rabbit polyclonal to Staufen
  • Tested applicationsSuitable for: ICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Horse, Cow, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 - 450 of Human Staufen.

    (Peptide available as ab73725.)

  • Positive control
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: SK N BE, SW480



Our Abpromise guarantee covers the use of ab73478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
IHC-P Use a concentration of 5 µg/ml.


  • FunctionBinds double-stranded RNA (regardless of the sequence) and tubulin. May play a role in specific positioning of mRNAs at given sites in the cell by cross-linking cytoskeletal and RNA components, and in stimulating their translation at the site.
  • Tissue specificityWidely expressed. Expressed in brain, pancreas, heart, skeletal muscles, liver, lung, kidney and placenta.
  • Sequence similaritiesContains 3 DRBM (double-stranded RNA-binding) domains.
  • DomainOne of the DRDB could be involved in RER binding.
    The C-terminal contains the tubulin binding domain (TBD).
  • Cellular localizationCytoplasm. Rough endoplasmic reticulum. Localizes exclusively with the rough reticulum endoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • Double stranded RNA binding protein Staufen homolog 1 antibody
    • Double stranded RNA binding protein Staufen homolog antibody
    • Double-stranded RNA-binding protein Staufen homolog 1 antibody
    • FLJ25010 antibody
    • MGC124588 antibody
    • STAU antibody
    • STAU1 antibody
    • STAU1_HUMAN antibody
    • staufen antibody
    • Staufen RNA binding protein (Drosophila) antibody
    • Staufen RNA binding protein homolog 1 antibody
    • Staufen, RNA binding protein, homolog 1 (Drosophila) antibody
    see all

Anti-Staufen antibody images

  • All lanes : Anti-Staufen antibody (ab73478) at 1 µg/ml

    Lane 1 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 63 kDa
    Observed band size : 63 kDa
    Additional bands at : 95 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • Staufen was immunoprecipitated using 0.5mg SW480 whole cell extract, 5µg of Rabbit polyclonal to Staufen and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, SW480 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73478.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697)..
    Band: 63kDa: Staufen; non specific - 75kDa: We are unsure as to the identity of this extra band.
  • ICC/IF image of ab73478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) MCF7 cells at 5µg/ml.
  • IHC image of Staufen staining in Human Cervical Cancer FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73478, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References for Anti-Staufen antibody (ab73478)

This product has been referenced in:
  • Pettersson OJ  et al. DDX6 regulates sequestered nuclear CUG-expanded DMPK-mRNA in dystrophia myotonica type 1. Nucleic Acids Res 42:7186-200 (2014). Read more (PubMed: 24792155) »
  • Ravel-Chapuis A  et al. The RNA-binding protein Staufen1 impairs myogenic differentiation via a c-myc-dependent mechanism. Mol Biol Cell 25:3765-78 (2014). WB, IHC-Fr ; Mouse . Read more (PubMed: 25208565) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse fibroblast)
Permeabilization Yes - Tween-20
Specification Mouse fibroblast
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Nov 24 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 6.0 citric acid
Sample Mouse Tissue sections (kidney)
Specification kidney
Permeabilization No
Fixative Paraformaldehyde

Dr. Min young Kim

Verified customer

Submitted Apr 11 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Non-reduced Denaturing (10%)
Sample Mouse Cell lysate - whole cell (MEL)
Specification MEL
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 16 2013

This antibody only detects stau1.

Application Western blot
Sample Mouse Tissue lysate - whole (Ms Brain Ms Heart Ms Liver Ms Lung)
Loading amount 20 µg
Specification Ms Brain Ms Heart Ms Liver Ms Lung
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Mr. Antibody Solutions

Verified customer

Submitted May 19 2010