PermaGreen/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. PermaGreen/AP produces a strong green color. It is insoluble in alcohol and xylene solutions (both aliphatic and hydrocarbon based) therefore sections can be dehydrated in alcohol, cleared in xylene substitute and permenently mounted.
Aliquot 3ml of StayGreen/AP substrate buffer in a mixing bottle. Add 20µl (one drop) of concentrated StayGreen/AP chromogen. Replace tip, mix and allow the solution to reach room temperature before using.
Note: The working chromagen-substrate solution should be prepared fresh and used withing 1 day of preparation. Any solution not used during this peeriod should be discarded.
Note: Increasing incubation temperature to 37°C will increase sensitivity and decrease needed incubation time.
Note: Use increasing concentrations of ethanol (up to 100%) to dehydrate. Use xylene substitute instead of xylene.
|Clear StayGreen/ AP Substrate Buffer||—|
|Concentrated StayGreen/AP Chromogen||—|
|Empty Mixing Dropping Bottle||—|
ab103745 has not yet been referenced specifically in any publications.