StayRed/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. StayRed/AP produces a brilliant dark red color. It is also insoluble in organic solvents so sections can be dehydrated in alcohol, cleared in xylene (or a xylene substitute) and permenently mounted.
Substrate/chromogen in conjunction with alkaline phosphatase-based immunostaining or in situ hybridization systems.
Aliquot 3mL of StayRed/AP Substrate Buffer in a mixing bottle. Add one drop (~20μL) of StayRed/AP Chromogen. Replace tip, mix, and allow solution to reach room temperature before using.
Note: The working chromogen-substrate solution should be prepared fresh and used within 20-30 minutes of preparation. Any solution not used during this period should be discarded.
|Empty Mixing Dropping Bottle||1 unit|
|StayRed/AP Chromogen||1 x 1ml|
|StayRed/AP Substrate Buffer||1 x 30ml|
Formalin fixed paraffin embedded Human tonsil stained with HMW Cytokeratin antibody (ab850) labeled with PermaRed/AP Chromogen (ab103741) produces a brilliant red color.