StayRed/AP 30ml (Alcohol & Xylene Compatible) (ab103741)

Overview

  • Product nameStayRed/AP 30ml (Alcohol & Xylene Compatible)
  • Assay typeDirect
  • Product overview

    StayRed/AP is a substrate chromogen system designed to be used for either IHC or ISH when using alkaline phosphate detection. StayRed/AP produces a brilliant dark red color. It is also insoluble in organic solvents so sections can be dehydrated in alcohol, cleared in xylene (or a xylene substitute) and permenently mounted.

  • Notes

    Intended Use:

    Substrate/chromogen in conjunction with alkaline phosphatase-based immunostaining or in situ hybridization systems.

     

    Working Solution:

    Aliquot 3mL of StayRed/AP Substrate Buffer in a mixing bottle. Add one drop (~20μL) of StayRed/AP Chromogen. Replace tip, mix, and allow solution to reach room temperature before using.

    Note: The working chromogen-substrate solution should be prepared fresh and used within 20-30 minutes of preparation. Any solution not used during this period should be discarded.

     

    Procedure:

    • After SA-alkaline phosphatase incubation, wash tissue sections with wash buffer.
    • Wipe slides, removing excess buffer. Add enough drops of StayRed/AP working solution to cover tissue sections.
    • Incubate for 5-15 minutes at room temperature. For optimal results, observe reaction under microscope for signal development. Once desired signal to noise ratio is achieved, stop reaction by rinsing slides in wash buffer.
    • Counterstain sections in hematoxylin.
    • Dehydrate sections in alcohol, clear in xylene (or xylene-substitute), and mount in permanent mounting medium.

Properties

    StayRed/AP 30ml (Alcohol & Xylene Compatible) images

    • Formalin fixed paraffin embedded Human tonsil stained with HMW Cytokeratin antibody (ab850) labeled with PermaRed/AP Chromogen (ab103741) produces a brilliant red color.

    • StayRed staining by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

    References for StayRed/AP 30ml (Alcohol & Xylene Compatible) (ab103741)

    This product has been referenced in:
    • Shami A  et al. Fibromodulin deficiency reduces low-density lipoprotein accumulation in atherosclerotic plaques in apolipoprotein E-null mice. Arterioscler Thromb Vasc Biol 33:354-61 (2013). Read more (PubMed: 23202368) »

    See 1 Publication for this product

    Product Wall

    Thank you for your inquiry.

    I can suggest to use a strepavidin that is coupled to an enzyme, for example: ab128985 (Streptavidin Peroxidase (Ready-To-Use)) and combine this with a suitable chromogen, for example: ab94665 (DAB substrate kit) <...

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    The source of StayRed and StayBlue recommend to do sequential staining. Their recommendations on chromogens for double staining are DAB and StayRed (http://www.abcam.com/ab103741). In order to use these chromogens first use DAB, then RED, followed...

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    Thank you for your enquiry and your interest in our products. I can confirm that this product (ab103741) works as double stain with HRP/DAB and it is suitable with Alkaline phosphatase detection. It may be worth first developing DAB and then Red si...

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    Thank you for your enquiry. I can confirm that these products can be used together for double staining. However, I can recommend to consider the following: 1. We would suggest to ensure the primary antibodies used in the stains undergo the sa...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"