Overview

  • Product nameAnti-STCH antibody
    See all STCH primary antibodies
  • Description
    Mouse monoclonal to STCH
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human, Recombinant Fragment
    Predicted to work with: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey
  • Immunogen

    Recombinant fragment: EDLFQKILVP IQQVLKEGHL EKTEIDEVVL VGGSTRIPRI RQVIQEFFGK DPNTSVDPDL AVVTGVAIQA GIDGGSWPLQ VSALEIPNKH LQKTNF, corresponding to amino acids 375-471 of Human STCH (NP_008879) with tag.

  • Positive control
    • NIH/3T3 cell lysate. Recombinant tagged human STCH fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77301 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 52 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged STCH is approximately 1ng/ml when used as a capture antibody.

Target

Anti-STCH antibody images

  • Anti-STCH antibody (ab77301) at 5 µg/ml + Recombinant tagged human STCH fragment at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 52 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • Anti-STCH antibody (ab77301) at 5 µg/ml + NIH/3T3 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 52 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)

References for Anti-STCH antibody (ab77301)

ab77301 has not yet been referenced specifically in any publications.

Product Wall

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"