STEAP1 overexpression 293T lysate (whole cell) (ab94255)

Overview

  • Product nameSTEAP1 overexpression 293T lysate (whole cell)
  • Description
    STEAP1 overexpression lysate
  • General notesab94255 is a 293T cell transfected lysate in which Human STEAP1 has been transiently over-expressed using a pCMV-STEAP1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor. Tissue specificity: Ubiquitously expressed. Highly expressed in prostate tumors. Similarity: Belongs to the STEAP family. Contains 1 ferric oxidoreductase domain.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94255 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

STEAP1 overexpression 293T lysate (whole cell) images

  • ab94255 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-STEAP1 antibody (ab57147) at 1/500 dilution

    Lane 1 : STEAP1 overexpression 293T lysate (whole cell) (ab94255)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for STEAP1 overexpression 293T lysate (whole cell) (ab94255)

ab94255 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"