Overview

  • Product name
  • Description
    Rabbit polyclonal to STEAP2
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human STEAP2 aa 233-262 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8NFT2

  • Positive control
    • 293 cell lysate; mouse spleen and liver tissue lysates; Human prostate carcinoma tissue; 293 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174978 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 56 kDa.
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor.
  • Tissue specificity
    Expressed at high levels in prostate and at significantly lower levels in heart, brain, kidney, pancreas, and ovary.
  • Sequence similarities
    Belongs to the STEAP family.
    Contains 1 ferric oxidoreductase domain.
  • Cellular localization
    Endosome membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4921538B17Rik antibody
    • AI930049 antibody
    • IPCA 1 antibody
    • IPCA1 antibody
    • Metalloreductase STEAP2 antibody
    • PCANAP 1 antibody
    • PCANAP1 antibody
    • Prostate cancer associated protein 1 antibody
    • Prostate cancer-associated protein 1 antibody
    • Protein up regulated in metastatic prostate cancer antibody
    • Protein upregulated in metastatic prostate cancer antibody
    • PUMPCn antibody
    • Six transmembrane epithelial antigen of prostate 2 antibody
    • Six transmembrane epithelial antigen of the prostate 2 antibody
    • Six-transmembrane epithelial antigen of prostate 2 antibody
    • SixTransMembrane protein of prostate 1 antibody
    • STAMP 1 antibody
    • STAMP1 antibody
    • STEA2_HUMAN antibody
    • STEAP 2 antibody
    • STEAP family member 2, metalloreductase antibody
    • Steap2 antibody
    • STMP antibody
    • UNQ6507/PRO23203 antibody
    see all

Images

  • All lanes : Anti-STEAP2 antibody (ab174978) at 1/100 dilution

    Lane 1 : Mouse spleen tissue lysate
    Lane 2 : Mouse liver tissue lysate
    Lane 3 : 293 cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 56 kDa
  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling STEAP2 with ab174978 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow cytometry analysis of 293 cells labeling STEAP2 with ab174978 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab174978 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174978.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up