Overview

  • Product name
    Anti-STEAP2 antibody
    See all STEAP2 primary antibodies
  • Description
    Rabbit polyclonal to STEAP2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human STEAP2 aa 233-262 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8NFT2

  • Positive control
    • 293 cell lysate; mouse spleen and liver tissue lysates; Human prostate carcinoma tissue; 293 cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174978 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 56 kDa.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Metalloreductase that has the ability to reduce both Fe(3+) to Fe(2+) and Cu(2+) to Cu(1+). Uses NAD(+) as acceptor.
  • Tissue specificity
    Expressed at high levels in prostate and at significantly lower levels in heart, brain, kidney, pancreas, and ovary.
  • Sequence similarities
    Belongs to the STEAP family.
    Contains 1 ferric oxidoreductase domain.
  • Cellular localization
    Endosome membrane. Cell membrane.
  • Information by UniProt
  • Database links
    • Alternative names
      • 4921538B17Rik antibody
      • AI930049 antibody
      • IPCA 1 antibody
      • IPCA1 antibody
      • Metalloreductase STEAP2 antibody
      • PCANAP 1 antibody
      • PCANAP1 antibody
      • Prostate cancer associated protein 1 antibody
      • Prostate cancer-associated protein 1 antibody
      • Protein up regulated in metastatic prostate cancer antibody
      • Protein upregulated in metastatic prostate cancer antibody
      • PUMPCn antibody
      • Six transmembrane epithelial antigen of prostate 2 antibody
      • Six transmembrane epithelial antigen of the prostate 2 antibody
      • Six-transmembrane epithelial antigen of prostate 2 antibody
      • SixTransMembrane protein of prostate 1 antibody
      • STAMP 1 antibody
      • STAMP1 antibody
      • STEA2_HUMAN antibody
      • STEAP 2 antibody
      • STEAP family member 2, metalloreductase antibody
      • Steap2 antibody
      • STMP antibody
      • UNQ6507/PRO23203 antibody
      see all

    Images

    • Western blot - Anti-STEAP2 antibody (ab174978)
      Western blot - Anti-STEAP2 antibody (ab174978)
      All lanes : Anti-STEAP2 antibody (ab174978) at 1/100 dilution

      Lane 1 : Mouse spleen tissue lysate
      Lane 2 : Mouse liver tissue lysate
      Lane 3 : 293 cell lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 56 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody (ab174978)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STEAP2 antibody (ab174978)

      Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human prostate carcinoma tissue labeling STEAP2 with ab174978 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

    • Flow Cytometry - Anti-STEAP2 antibody (ab174978)
      Flow Cytometry - Anti-STEAP2 antibody (ab174978)

      Flow cytometry analysis of 293 cells labeling STEAP2 with ab174978 at 1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

    References

    ab174978 has not yet been referenced specifically in any publications.

    Publishing research using ab174978? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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