• Product nameAnti-STEAP3 antibody
    See all STEAP3 primary antibodies
  • Description
    Rabbit polyclonal to STEAP3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse STEAP3.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Liver; Mouse Brain; Mouse Heart. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: JEG3.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab104654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF Use a concentration of 1 µg/ml.


  • FunctionEndosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.
  • Tissue specificityExpressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.
  • Involvement in diseaseAnemia, hypochromic microcytic, with iron overload 2
  • Sequence similaritiesBelongs to the STEAP family.
    Contains 1 ferric oxidoreductase domain.
  • Post-translational
    Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.
  • Cellular localizationEndosome membrane. Localizes to vesicular-like structures at the plasma membrane and around the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1010001D01Rik antibody
    • Dudlin 2 antibody
    • Dudlin2 antibody
    • Dudulin 2 antibody
    • Dudulin-2 antibody
    • Dudulin2 antibody
    • hpHyde antibody
    • hTSAP6 antibody
    • Metalloreductase STEAP3 antibody
    • MGC93147 antibody
    • pHyde antibody
    • Six transmembrane epithelial antigen of prostate 3 antibody
    • Six transmembrane prostate protein 3 antibody
    • Six-transmembrane epithelial antigen of prostate 3 antibody
    • STEA 3 antibody
    • STEA3_HUMAN antibody
    • STEAP 3 antibody
    • STEAP family member 3 antibody
    • STEAP family member 3 metalloreductase antibody
    • STEAP3 antibody
    • STMP 3 antibody
    • STMP3 antibody
    • TSAP 6 antibody
    • TSAP6 antibody
    • Tumor suppressor activated pathway protein 6 antibody
    • Tumor suppressor pHyde antibody
    • Tumor suppressor-activated pathway protein 6 antibody
    see all

Anti-STEAP3 antibody images

  • All lanes : Anti-STEAP3 antibody (ab104654) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 105 kDa,130 kDa,46 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab104654 stained JEG3 cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104654 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-STEAP3 antibody (ab104654)

ab104654 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab104654.
Please use the links above to contact us or submit feedback about this product.