Overview

  • Product name
  • Description
    Rabbit polyclonal to STEAP3
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse STEAP3.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse Liver; Mouse Brain; Mouse Heart. This antibody gave a positive result in IF in the following formaldehyde fixed cell lines: JEG3.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104654 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • Function
    Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.
  • Tissue specificity
    Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.
  • Involvement in disease
    Anemia, hypochromic microcytic, with iron overload 2
  • Sequence similarities
    Belongs to the STEAP family.
    Contains 1 ferric oxidoreductase domain.
  • Post-translational
    modifications
    Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.
    Glycosylated.
  • Cellular localization
    Endosome membrane. Localizes to vesicular-like structures at the plasma membrane and around the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1010001D01Rik antibody
    • Dudlin 2 antibody
    • Dudlin2 antibody
    • Dudulin 2 antibody
    • Dudulin-2 antibody
    • Dudulin2 antibody
    • hpHyde antibody
    • hTSAP6 antibody
    • Metalloreductase STEAP3 antibody
    • MGC93147 antibody
    • pHyde antibody
    • Six transmembrane epithelial antigen of prostate 3 antibody
    • Six transmembrane prostate protein 3 antibody
    • Six-transmembrane epithelial antigen of prostate 3 antibody
    • STEA 3 antibody
    • STEA3_HUMAN antibody
    • STEAP 3 antibody
    • STEAP family member 3 antibody
    • STEAP family member 3 metalloreductase antibody
    • STEAP3 antibody
    • STMP 3 antibody
    • STMP3 antibody
    • TSAP 6 antibody
    • TSAP6 antibody
    • Tumor suppressor activated pathway protein 6 antibody
    • Tumor suppressor pHyde antibody
    • Tumor suppressor-activated pathway protein 6 antibody
    see all

Images

  • All lanes : Anti-STEAP3 antibody (ab104654) at 1 µg/ml

    Lane 1 : Liver (Mouse) Tissue Lysate
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 55 kDa
    Additional bands at : 105 kDa,130 kDa,46 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
  • ICC/IF image of ab104654 stained JEG3 cells. The cells were 4% formaldehyde fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab104654 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab104654 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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