Overview

  • Product nameAnti-STEAP3 antibody
    See all STEAP3 primary antibodies
  • Description
    Rabbit polyclonal to STEAP3
  • SpecificityDetects endogenous levels of total STEAP3 protein.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human STEAP3

  • Positive control
    • COLO205 cell extract HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab72513 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.

Target

  • FunctionEndosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.
  • Tissue specificityExpressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.
  • Involvement in diseaseAnemia, hypochromic microcytic, with iron overload 2
  • Sequence similaritiesBelongs to the STEAP family.
    Contains 1 ferric oxidoreductase domain.
  • Post-translational
    modifications
    Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.
    Glycosylated.
  • Cellular localizationEndosome membrane. Localizes to vesicular-like structures at the plasma membrane and around the nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1010001D01Rik antibody
    • Dudlin 2 antibody
    • Dudlin2 antibody
    • Dudulin 2 antibody
    • Dudulin-2 antibody
    • Dudulin2 antibody
    • hpHyde antibody
    • hTSAP6 antibody
    • Metalloreductase STEAP3 antibody
    • MGC93147 antibody
    • pHyde antibody
    • Six transmembrane epithelial antigen of prostate 3 antibody
    • Six transmembrane prostate protein 3 antibody
    • Six-transmembrane epithelial antigen of prostate 3 antibody
    • STEA 3 antibody
    • STEA3_HUMAN antibody
    • STEAP 3 antibody
    • STEAP family member 3 antibody
    • STEAP family member 3 metalloreductase antibody
    • STEAP3 antibody
    • STMP 3 antibody
    • STMP3 antibody
    • TSAP 6 antibody
    • TSAP6 antibody
    • Tumor suppressor activated pathway protein 6 antibody
    • Tumor suppressor pHyde antibody
    • Tumor suppressor-activated pathway protein 6 antibody
    see all

Anti-STEAP3 antibody images

  • All lanes : Anti-STEAP3 antibody (ab72513) at 1/500 dilution

    Lane 1 : COLO205 cell extract
    Lane 2 : COLO205 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 55 kDa
    Observed band size : 55 kDa
  • Immunofluorescence analysis of HeLa cells, using ab72513 at 1/500 dilution. Right panel was also treated with immunising peptide.

References for Anti-STEAP3 antibody (ab72513)

This product has been referenced in:
  • Ma YP & Zhu WL Cytoplasmic and nuclear localization of TCTP in normal and cancer cells. Biochem Res Int 2012:871728 (2012). ICC/IF ; Human . Read more (PubMed: 22666592) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"