Recombinant
RabMAb

Anti-STEAP3 antibody [EPR9812] (ab151566)

Overview

  • Product name
    Anti-STEAP3 antibody [EPR9812]
    See all STEAP3 primary antibodies
  • Description
    Rabbit monoclonal [EPR9812] to STEAP3
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HeLa, HepG2, PC12 and C6 cell lysates; HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab151566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 54 kDa.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Function
      Endosomal ferrireductase required for efficient transferrin-dependent iron uptake in erythroid cells. Participates in erythroid iron homeostasis by reducing Fe(3+) to Fe(2+). Can also reduce of Cu(2+) to Cu(1+), suggesting that it participates in copper homeostasis. Uses NADP(+) as acceptor. May play a role downstream of p53/TP53 to interface apoptosis and cell cycle progression. Indirectly involved in exosome secretion by facilitating the secretion of proteins such as TCTP.
    • Tissue specificity
      Expressed in adult bone marrow, placenta, liver, skeletal muscle and pancreas. Down-regulated in hepatocellular carcinoma.
    • Involvement in disease
      Anemia, hypochromic microcytic, with iron overload 2
    • Sequence similarities
      Belongs to the STEAP family.
      Contains 1 ferric oxidoreductase domain.
    • Post-translational
      modifications
      Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage occurs at multiple sites in a glycosylation-independent manner.
      Glycosylated.
    • Cellular localization
      Endosome membrane. Localizes to vesicular-like structures at the plasma membrane and around the nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 1010001D01Rik antibody
      • Dudlin 2 antibody
      • Dudlin2 antibody
      • Dudulin 2 antibody
      • Dudulin-2 antibody
      • Dudulin2 antibody
      • hpHyde antibody
      • hTSAP6 antibody
      • Metalloreductase STEAP3 antibody
      • MGC93147 antibody
      • pHyde antibody
      • Six transmembrane epithelial antigen of prostate 3 antibody
      • Six transmembrane prostate protein 3 antibody
      • Six-transmembrane epithelial antigen of prostate 3 antibody
      • STEA 3 antibody
      • STEA3_HUMAN antibody
      • STEAP 3 antibody
      • STEAP family member 3 antibody
      • STEAP family member 3 metalloreductase antibody
      • STEAP3 antibody
      • STMP 3 antibody
      • STMP3 antibody
      • TSAP 6 antibody
      • TSAP6 antibody
      • Tumor suppressor activated pathway protein 6 antibody
      • Tumor suppressor pHyde antibody
      • Tumor suppressor-activated pathway protein 6 antibody
      see all

    Images

    • All lanes : Anti-STEAP3 antibody [EPR9812] (ab151566) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : PC12 cell lysate
      Lane 4 : C6 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 54 kDa
    • Immunofluorescent analysis of HepG2 cells labeling STEAP3 with ab151566 at 1/250 dilution.

    References

    ab151566 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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