Anti-Cystatin-B antibody [2F1] (ab72353)
Key features and details
- Mouse monoclonal [2F1] to Cystatin-B
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-Cystatin-B antibody [2F1]
See all Cystatin-B primary antibodies -
Description
Mouse monoclonal [2F1] to Cystatin-B -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human Cystatin-B.
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Positive control
- A549 cell lysate
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General notes
This product was changed from ascites to tissue culture supernatant on 08/05/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
2F1 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
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ICC/IF |
1/100.
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Notes |
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WB
Use at an assay dependent concentration. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa). |
ICC/IF
1/100. |
Target
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Function
This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B. -
Involvement in disease
Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) [MIM:254800]. EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop. -
Sequence similarities
Belongs to the cystatin family. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1476 Human
- Omim: 601145 Human
- SwissProt: P04080 Human
- Unigene: 695 Human
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Alternative names
- CHROW21 antibody
- CPI B antibody
- CPI-B antibody
see all
Images
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ICC/IF analysis of CSTB in A549 cells. The cell was stained with ab72353(1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
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All lanes : Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : U87MG cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 11 kDa -
Anti-Cystatin-B antibody [2F1] (ab72353) at 1/1000 dilution + A549 cells lysate at 35 µg
Secondary
goat anti-mouse secondary antibody conjugated to HRP
Developed using the ECL technique.
Predicted band size: 11 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab72353 has been referenced in 1 publication.
- Burgener A et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol 87:5141-50 (2013). Human . PubMed: 23449785