Overview

  • Product name
    Anti-Stefin B antibody [2F1]
    See all Stefin B primary antibodies
  • Description
    Mouse monoclonal [2F1] to Stefin B
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human Stefin B (1-98 aa) purified from E. coli

  • Positive control
    • A549 cell lysate
  • General notes


    is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Stefin B protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Stefin B protein.
  • Clonality
    Monoclonal
  • Clone number
    2F1
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/3000. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
ELISA Use at an assay dependent dilution.

Target

  • Function
    This is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
  • Involvement in disease
    Defects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) [MIM:254800]. EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop.
  • Sequence similarities
    Belongs to the cystatin family.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CHROW21 antibody
    • CPI B antibody
    • CPI-B antibody
    • CST 6 antibody
    • CST6 antibody
    • CSTB antibody
    • Cystatin B (stefin B) antibody
    • Cystatin B antibody
    • Cystatin-B antibody
    • CYTB antibody
    • CYTB_HUMAN antibody
    • EPM1 antibody
    • EPM1A antibody
    • Liver thiol proteinase inhibitor antibody
    • PME antibody
    • Stefin-B antibody
    • STF B antibody
    • STFB antibody
    • ULD antibody
    see all

Images

  • Anti-Stefin B antibody [2F1] (ab72353) at 1/1000 dilution + A549 cells lysate at 35 µg

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 11 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Burgener A  et al. A systems biology examination of the human female genital tract shows compartmentalization of immune factor expression. J Virol 87:5141-50 (2013). Human . Read more (PubMed: 23449785) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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