Anti-Stefin B antibody [EPR3931] (ab92449)


  • Product nameAnti-Stefin B antibody [EPR3931]
    See all Stefin B primary antibodies
  • Description
    Rabbit monoclonal [EPR3931] to Stefin B
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • U87-MG, JAR, and Human bladder cell lysates; Human colon tissue; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.


Associated products


Our Abpromise guarantee covers the use of ab92449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notesFlow Cyt: 1/10 - 1/100.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended for enhanced staining.
    WB: 1/1000 - 1/10000. Predicted molecular weight: 11 kDa.

    Is unsuitable for ICC or IP.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionThis is an intracellular thiol proteinase inhibitor. Tightly binding reversible inhibitor of cathepsins L, H and B.
    • Involvement in diseaseDefects in CSTB are the cause of progressive myoclonic epilepsy type 1 (EPM1) [MIM:254800]. EPM1 is an autosomal recessive disorder characterized by severe, stimulus-sensitive myoclonus and tonic-clonic seizures. The onset, occurring between 6 and 13 years of age, is characterized by convulsions. Myoclonus begins 1 to 5 years later. The twitchings occur predominantly in the proximal muscles of the extremities and are bilaterally symmetrical, although asynchronous. At first small, they become late in the clinical course so violent that the victim is thrown to the floor. Mental deterioration and eventually dementia develop.
    • Sequence similaritiesBelongs to the cystatin family.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CHROW21 antibody
      • CPI B antibody
      • CPI-B antibody
      • CST 6 antibody
      • CST6 antibody
      • CSTB antibody
      • Cystatin B (stefin B) antibody
      • Cystatin B antibody
      • Cystatin-B antibody
      • CYTB antibody
      • CYTB_HUMAN antibody
      • EPM1 antibody
      • EPM1A antibody
      • Liver thiol proteinase inhibitor antibody
      • PME antibody
      • Stefin-B antibody
      • STF B antibody
      • STFB antibody
      • ULD antibody
      see all

    Anti-Stefin B antibody [EPR3931] images

    • All lanes : Anti-Stefin B antibody [EPR3931] (ab92449) at 1/1000 dilution

      Lane 1 : U87-MG cell lysate
      Lane 2 : JAR cell lysate
      Lane 3 : Human bladder lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 11 kDa
    • ab92449, at 1/100 dilution, staining Stefin B in paraffin-embedded Human colon tissue.
    • ab92449, at 1/100 dilution, staining Stefin B in paraffin-embedded Human kidney tissue.

    References for Anti-Stefin B antibody [EPR3931] (ab92449)

    ab92449 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your reply and for spotting my mistake. ab92449 is indeed described as not suitable for mouse samples. So, as requested, I have issued a free of charge replacement with ab54566. The order number is 954882. To check the stat...

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    Thank you for your reply and those precisions. We have an alternative anti-CSTB, ab92449, directed against the C-terminal region of the human protein (between amino acids 73 and 98). The homology between the region...

    Read More