Overview

  • Product nameAnti-Stella antibody
    See all Stella primary antibodies
  • Description
    Rabbit polyclonal to Stella
  • Tested applicationsSuitable for: IHC-Fr, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Mouse Stella.

    (Peptide available as ab23324.)

  • Positive control
    • This antibody gave a positive signal for Western Blot in Mouse Embryonic Germ Cell, Embryonic Stem Cell and Embryonic Carcinoma Cell Lysates. This antibody gave a positive signal for Immunofluorescence in Mouse Embryonic Stem cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab19878 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 4 µg/ml.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionMay play a role in maintaining cell pluripotentiality.
  • Tissue specificityLow expression in testis, ovary and thymus. Expressed in embryonic stem and carcinoma cells. Highly expressed in testicular germ cell tumors.
  • Developmental stageExpressed in fetal ovary.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • FormStella is a gene specifically expressed in primordial germ cells, oocytes, preimplantation embryos and pluripotent cells. It encodes a protein with a SAP-like domain and a splicing factor motif-like structure, suggesting possible roles in chromosomal organization or RNA processing. Embryos without Stella are compromised in preimplantation development and rarely reach the blastocyst stage.
  • Alternative names
    • Compaction associated protein 1 antibody
    • Developmental pluripotency associated 3 antibody
    • Developmental pluripotency associated protein 3 antibody
    • Developmental pluripotency-associated protein 3 antibody
    • Dppa3 antibody
    • DPPA3_HUMAN antibody
    • PGC7 antibody
    • Stella related protein antibody
    • Stella-related protein antibody
    see all

Anti-Stella antibody images

  • The image shows ab19878 staining of a crysosection of mouse embryonic genital ridges (E14.5). The samples were fixed overnight in 4% paraformaldehyde, permeabilised with 0.1% Triton and stained overnight at 4 degrees. 4µg/ml of antibody was used. Staining was in the nucleus and cytoplasm of Oct4-positive cells. The blue fluorescence is DAPI staining of DNA.

  • All lanes : Anti-Stella antibody (ab19878) at 1 µg/ml

    Lane 1 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
    Lane 2 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 3 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 4 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate with Mouse Stella peptide (ab23324) at 2 µg/ml
    Lane 5 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse Stella peptide (ab23324) at 2 µg/ml
    Lane 6 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate with Mouse Stella peptide (ab23324) at 2 µg/ml

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Predicted band size : 17 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 125 kDa,25 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ICC/IF image of ab19878 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • ICC/IF image of ab19878 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19878, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence analysis of porcine induced pluripotent stem cells cultured in 2i + LIF medium, staining Stella with ab19878.

    Cells were fixed in 4% paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked in 5% BSA. Cells were incubated with primary antibody (1/60) overnight at 4°C.

References for Anti-Stella antibody (ab19878)

This product has been referenced in:
  • Wang H  et al. Induction of Germ Cell-like Cells from Porcine Induced Pluripotent Stem Cells. Sci Rep 6:27256 (2016). ICC/IF ; Pig . Read more (PubMed: 27264660) »
  • Roper SJ  et al. ADP-ribosyltransferases Parp1 and Parp7 safeguard pluripotency of ES cells. Nucleic Acids Res 42:8914-27 (2014). ICC/IF ; Mouse . Read more (PubMed: 25034692) »

See all 10 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Testis, embryonic day 15.5)
Permeabilization Yes - 0.1% Triton X-100 in PBS
Specification Testis, embryonic day 15.5
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Mr. Bryan Niedenberger

Verified customer

Submitted Apr 06 2016

Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic stem cell)
Loading amount 200000 cells
Specification mouse embryonic stem cell
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Username

Abcam user community

Verified customer

Submitted Oct 24 2012

Thank you for contacting us.

Your credit note ID is XXXXX.

I am sorry that this antibody did not perform as stated on the datasheet, I have asked our Finance department to issue a credit note for you. The credit note may be used in on...

Read More

Thank you for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appr...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (embryonic stem cell)
Specification embryonic stem cell
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 0.1% · Temperature: 16°C
Username

Abcam user community

Verified customer

Submitted Oct 11 2012

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided; the protocol looks fine to me to however I have following further suggestions for impro...

Read More

Thank you for contacting us.

I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am at...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Adult ovary)
Specification Adult ovary
Fixative Formaldehyde
Permeabilization No
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

煜欽 林

Verified customer

Submitted Aug 29 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Marmoset (common) Tissue sections (Marmoset adult testis)
Specification Marmoset adult testis
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Target retrieval solution by Dako
Permeabilization Yes - Triton-X
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

煜欽 林

Verified customer

Submitted Aug 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Embyro)
Specification Embyro
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Username

Abcam user community

Verified customer

Submitted Nov 14 2006

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"