The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution. Detection limit dilution for peptide ELISA: 1/64000.
WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa.
Preliminary experiments gave an approx 26kDa band in Human ovary and placenta lysates after 0.1µg/ml antibody staining. The band was successfully blocked by incubation with the immunizing peptide.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceSTELLAR is a germ and embryonic stem cell enriched protein STELLA.