Anti-STEP / PTPN5 antibody (ab155329)

Overview

  • Product name
    Anti-STEP / PTPN5 antibody
    See all STEP / PTPN5 primary antibodies
  • Description
    Rabbit polyclonal to STEP / PTPN5
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 347 - 565 of Human STEP/ PTPN5 (UniProt ID: P54829).

  • Positive control
    • 293T and HepG2 cell lysate; mouse brain lysate; A549 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 64 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris/EDTA buffer (pH 8.0) may also be used.

Target

  • Function
    May regulates the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Phosphorylation at Ser-245 by PKA deactivates PTPN5. Phosphorylation at Thr-255 and Ser-268 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ14427 antibody
    • Neural specific protein tyrosine phosphatase antibody
    • Neural-specific protein-tyrosine phosphatase antibody
    • Protein tyrosine phosphatase non receptor type 5 (striatum enriched) antibody
    • Protein tyrosine phosphatase non receptor type 5 antibody
    • Protein tyrosine phosphatase striatum enriched antibody
    • PTN5 antibody
    • PTN5_HUMAN antibody
    • PTP STEP antibody
    • PTPN 5 antibody
    • Ptpn5 antibody
    • PTPSTEP antibody
    • STEP antibody
    • Striatum-enriched protein-tyrosine phosphatase antibody
    • Tyrosine protein phosphatase non receptor type 5 antibody
    • Tyrosine-protein phosphatase non-receptor type 5 antibody
    see all

Images

  • Anti-STEP / PTPN5 antibody (ab155329) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size : 64 kDa
  • Anti-STEP / PTPN5 antibody (ab155329) at 1/1000 dilution + mouse brain lysate at 50 µg

    Predicted band size : 64 kDa
  • Immunohistochemical analysis of paraffin-embedded A549 xenograft tissue labeling STEP/PTPN5 with ab155329 at 1/500 dilution.

References

ab155329 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (8)
Sample
Zebra finch Cell lysate - whole cell (HeK cells overexpressing zebra finch PTPN5)
Specification
HeK cells overexpressing zebra finch PTPN5
Blocking step
Rotiblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

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Verified customer

Submitted Jan 10 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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