Overview

  • Product nameAnti-STEP / PTPN5 antibody [T41]
    See all STEP / PTPN5 primary antibodies
  • Description
    Mouse monoclonal [T41] to STEP / PTPN5
  • Tested applicationsSuitable for: WB, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant full length Human protein, purified from E. Coli

  • Positive control
    • IMR-32 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IP Use at an assay dependent concentration. PubMed: 20959805
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionMay regulates the activity of several effector molecules involved in synaptic plasticity and neuronal cell survival, including MAPKs, Src family kinases and NMDA receptors.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Post-translational
    modifications
    Phosphorylation at Ser-245 by PKA deactivates PTPN5. Phosphorylation at Thr-255 and Ser-268 by MAPKs stabilizes the phosphatase, dephosphorylation of these sites results in ubiquitin-mediated degradation of the active phosphatase.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ14427 antibody
    • Neural specific protein tyrosine phosphatase antibody
    • Neural-specific protein-tyrosine phosphatase antibody
    • Protein tyrosine phosphatase non receptor type 5 (striatum enriched) antibody
    • Protein tyrosine phosphatase non receptor type 5 antibody
    • Protein tyrosine phosphatase striatum enriched antibody
    • PTN5 antibody
    • PTN5_HUMAN antibody
    • PTP STEP antibody
    • PTPN 5 antibody
    • Ptpn5 antibody
    • PTPSTEP antibody
    • STEP antibody
    • Striatum-enriched protein-tyrosine phosphatase antibody
    • Tyrosine protein phosphatase non receptor type 5 antibody
    • Tyrosine-protein phosphatase non-receptor type 5 antibody
    see all

Anti-STEP / PTPN5 antibody [T41] images

  • Anti-STEP / PTPN5 antibody [T41] (ab77123) at 1/1000 dilution + IMR-32 cell lysate

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
  • Overlay histogram showing HepG2 cells stained with ab77123 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77123, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-STEP / PTPN5 antibody [T41] (ab77123)

This product has been referenced in:

See 1 Publication for this product

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