Anti-Sterol carrier protein 2 antibody (ab67694)

Overview

  • Product name
    Anti-Sterol carrier protein 2 antibody
    See all Sterol carrier protein 2 primary antibodies
  • Description
    Mouse polyclonal to Sterol carrier protein 2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein corresponding to Human Sterol carrier protein 2 aa 1-322.
    Sequence:

    MSSSPWEPATLRRVFVVGVGMTKFVKPGAENSRDYPDLAEEAGKKALADA QIPYSAVDQACVGYVFGVAECVLALGFEKMSKGSLGIKFSDRTIPTDKHV DLLINKYGLSAHPVAPQMFGYAGKEHMEKYGTKIEHFAKIGWKNHKHSVN NPYSQFQDEYSLDEVMASKEVFDFLTILQCCPTSDGAAAAILASEAFVQK YGLQSKAVEILAQEMMTDLPSSFEEKSIIKMVGFDMSKEAARKCYEKSGL TPNDIDVIELHDCFSTNELLTYEALGLCPEGQGATLVDRGDNTYGGKWVI NPSGGLISKGHPLGATGGHSCS


    Database link: P22307-3

  • Positive control
    • Sterol carrier protein 2 transfected 293T cell lysate and human liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab67694 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Predicted molecular weight: 175 kDa.

Target

  • Function
    Mediates in vitro the transfer of all common phospholipids, cholesterol and gangliosides between membranes. May play a role in regulating steroidogenesis.
  • Tissue specificity
    Liver, fibroblasts, and placenta.
  • Involvement in disease
    Defects in SCP2 are a cause of leukoencephalopathy with dystonia and motor neuropathy (LDMN) [MIM:613724]; also known as sterol carrier protein 2 deficiency. LDMN is a syndrome characterized by leukoencephalopathy, dystonic head tremor, spasmodic torticollis and reduced tendon reflexes in lower extremities. Additional features include hyposmia, pathologic saccadic eye movements, a slight hypoacusis, accumulation of branched-chain pristanic acid in plasma, and the presence of abnormal bile alcohol glucuronides in urine.
  • Sequence similarities
    In the N-terminal section; belongs to the thiolase family.
    Contains 1 SCP2 domain.
  • Cellular localization
    Mitochondrion; Cytoplasm. Mitochondrion. Cytoplasmic in the liver and also associated with mitochondria especially in steroidogenic tissues and Peroxisome. Interaction with PEX5 is essential for peroxisomal import.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686C12188 antibody
    • DKFZp686D11188 antibody
    • NLTP antibody
    • NLTP_HUMAN antibody
    • Non-specific lipid-transfer protein antibody
    • Nonspecific lipid transfer protein antibody
    • NSL TP antibody
    • NSL-TP antibody
    • OTTHUMP00000010488 antibody
    • OTTHUMP00000231766 antibody
    • OTTHUMP00000231767 antibody
    • OTTHUMP00000231768 antibody
    • OTTHUMP00000231769 antibody
    • OTTHUMP00000231770 antibody
    • OTTHUMP00000231772 antibody
    • OTTHUMP00000231773 antibody
    • OTTHUMP00000231774 antibody
    • OTTHUMP00000231776 antibody
    • OTTHUMP00000234662 antibody
    • Propanoyl CoA C acyltransferase antibody
    • Propanoyl-CoA C-acyltransferase antibody
    • SCP 2 antibody
    • SCP chi antibody
    • SCP X antibody
    • SCP-2 antibody
    • SCP-chi antibody
    • SCP-X antibody
    • SCP2 antibody
    • SCPchi antibody
    • SCPX antibody
    • Sterol carrier protein 2 antibody
    • Sterol carrier protein X antibody
    see all

Anti-Sterol carrier protein 2 antibody images

  • All lanes : Anti-Sterol carrier protein 2 antibody (ab67694) at 1/500 dilution

    Lane 1 : SCP2 transfected 293T cell lysate
    Lane 2 : Non 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 175 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • Anti-Sterol carrier protein 2 antibody (ab67694) at 1/500 dilution + human liver lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP conjugate at 1/2500 dilution

    Predicted band size : 175 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 63 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Sterol carrier protein 2 antibody (ab67694)

ab67694 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Spermatocyte)
Specification
Spermatocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.15% Triton X 100
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.1% · Temperature: 25°C
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Submitted Nov 18 2009

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