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Anti-STIP1 antibody images
IHC-P - STIP1 antibody (ab56873)
STIP1 antibody (ab56873) used in immunohistochemistry at 5ug/ml on formalin fixed and paraffin embedded human testis.
Western blot - STIP1 antibody (ab56873)
Predicted band size : 63 kDa STIP1 antibody (ab56873) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry-Anti-STIP1 antibody(ab56873)
Overlay histogram showing HeLa cells stained with ab56873 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56873, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-STIP1 antibody (ab56873)
This product has been referenced in:
El-Kasaby A et al. A cytosolic relay of heat shock proteins HSP70-1A and HSP90ß monitors the folding trajectory of the serotonin transporter. J Biol Chem289:28987-9000 (2014).
Read more (PubMed: 25202009) »
Xue L et al. Identification of direct tyrosine kinase substrates based on protein kinase assay-linked phosphoproteomics. Mol Cell Proteomics12:2969-80 (2013).
Read more (PubMed: 23793017) »