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- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 2% Sucrose, PBS
Concentration information loading...
- PurityProtein A purified
- Research areas
- Compatible Secondaries
- Positive Controls
Our Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1.25 µg/ml. Detects a band of approximately 59 kDa (predicted molecular weight: 63 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
||Use a concentration of 4 - 8 µg/ml.
- FunctionMediates the association of the molecular chaperones HSC70 and HSP90 (HSPCA and HSPCB).
- Sequence similaritiesContains 2 STI1 domains.
Contains 9 TPR repeats.
- DomainThe TPR 1 repeat interacts with the C-terminal of HSC70. The TPR 4, 5 and 6 repeats (also called TPR2A domain) and TPR 7, 8 and 9 repeats (also called TPR2B domain) interact with HSP90.
- Cellular localizationCytoplasm. Nucleus.
Information by UniProt
- Epididymis secretory sperm binding protein Li 94n antibody
- HEL S 94n antibody
- Hop antibody
Anti-STIP1 antibody images
Western blot - STIP1 antibody (ab65046)
Anti-STIP1 antibody (ab65046) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 63 kDa
Observed band size : 59 kDa (why is the actual band size different from the predicted?)
Antibody diluted in 5% skim milk / PBS buffer. Gel concentration 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - STIP1 antibody (ab65046)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling STIP1 with ab65046 at 4-8µg/ml. Arrows indicate positively labelled skeletal muscle cells. Magnification: 400X.
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