• Product nameAnti-STK3 antibody [3067C3a]
    See all STK3 primary antibodies
  • Description
    Mouse monoclonal [3067C3a] to STK3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment of human STK3

  • Positive control
    • Recombinant human STK3.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS (8.0mM Sodium phosphate, 3.0mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4)
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab71960 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane.
  • ClonalityMonoclonal
  • Clone number3067C3a
  • IsotypeIgG2b
  • Research areas


Our Abpromise guarantee covers the use of ab71960 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 37 kDa (predicted molecular weight: 56 kDa).


  • FunctionStress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.
  • Tissue specificityExpressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.
  • Cellular localizationCytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610042I06Rik antibody
    • EC antibody
    • FLJ90748 antibody
    • Kinase responsive to stress 1 antibody
    • KRS1 antibody
    • Mammalian STE20 like protein kinase 2 antibody
    • Mammalian STE20-like protein kinase 2 antibody
    • Mammalian sterile 20-like 2 antibody
    • Mess1 antibody
    • MST antibody
    • MST-2 antibody
    • MST2 antibody
    • Mst3 antibody
    • Serine/threonine kinase 3 (STE20 homolog, yeast) antibody
    • Serine/threonine kinase 3 (Ste20, yeast homolog) antibody
    • Serine/threonine protein kinase 3 antibody
    • Serine/threonine protein kinase Krs1 antibody
    • Serine/threonine-protein kinase 3 antibody
    • Serine/threonine-protein kinase Krs-1 antibody
    • STE20 like kinase MST2 antibody
    • STE20-like kinase MST2 antibody
    • Stk3 antibody
    • STK3_HUMAN antibody
    • wu:fc19e11 antibody
    • zgc:55383 antibody
    see all

Anti-STK3 antibody [3067C3a] images

  • Predicted band size : 56 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: STK3 knockout HAP1 cell lysate (20 µg)

    Lane 3: NIH/3T3 cell lysate (20 µg)
    Lane 4: 293T cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab71960 observed at 56 kDa. Red - loading control, ab181602, observed at 37 kDa.
    ab71960 was shown to recognize STK3 when STK3 knockout samples were used, along with additional cross-reactive bands. Wild-type and STK3 knockout samples were subjected to SDS-PAGE. ab71960 and ab181602 (loading control to STK3) were diluted 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

  • Anti-STK3 antibody [3067C3a] (ab71960) + immunising recombinant protein

    Predicted band size : 56 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.

References for Anti-STK3 antibody [3067C3a] (ab71960)

ab71960 has not yet been referenced specifically in any publications.

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