• Product name
  • Description
    Rabbit polyclonal to STK3
  • Host species
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Guinea pig, Cow, Turkey, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Gorilla, Tilapia, Orangutan, Xenopus tropicalis
  • Immunogen

    Synthetic peptide mapping to the C-terminus of Human STK3

  • Positive control
    • Whole cell lysate from HeLa, Ramos or NIH3T3 cells



Our Abpromise guarantee covers the use of ab70546 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000 - 1/15000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
IP Use at 2-5 µg/mg of lysate.


  • Function
    Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. MST1/MST2 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1.
  • Tissue specificity
    Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SARAH domain.
  • Cellular localization
    Cytoplasm. Nucleus. The caspase-cleaved form cycles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • 0610042I06Rik antibody
    • EC antibody
    • FLJ90748 antibody
    • KB 1458E12.1 antibody
    • Kinase responsive to stress 1 antibody
    • KRS1 antibody
    • Mammalian STE20 like protein kinase 2 antibody
    • Mammalian STE20-like protein kinase 2 antibody
    • Mammalian sterile 20-like 2 antibody
    • Mess1 antibody
    • MST antibody
    • MST-2 antibody
    • MST2 antibody
    • Mst3 antibody
    • Serine/threonine kinase 3 (STE20 homolog, yeast) antibody
    • Serine/threonine kinase 3 (Ste20, yeast homolog) antibody
    • Serine/threonine kinase 3 antibody
    • Serine/threonine protein kinase 3 antibody
    • Serine/threonine protein kinase Krs1 antibody
    • Serine/threonine-protein kinase 3 antibody
    • Serine/threonine-protein kinase Krs-1 antibody
    • STE20 like kinase MST2 antibody
    • STE20-like kinase MST2 antibody
    • Stk3 antibody
    • STK3_HUMAN antibody
    • wu:fc19e11 antibody
    • zgc:55383 antibody
    see all


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling STK3 with ab70546 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-STK3 antibody (ab70546) at 1/10000 dilution

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from Ramos cells at 50 µg
    Lane 5 : Whole cell lysate from NIH3T3 cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 56 kDa
    Observed band size: 56 kDa

    Exposure time: 3 seconds
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated with ab70546 at 3ug/mg of lysate (lane 1) or a control rabbit Ig )lane 2). For the subsequent blot, 25% of the immnuoprecipitate (lanes 1 and 2) or the input (lane 3) were probed with ab70546 at 1mg/ml.


ab70546 has not yet been referenced specifically in any publications.

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