Anti-STK3 (phospho T180) + MST1 (phospho T183) antibody (ab79199)

Overview

  • Product name
    Anti-STK3 (phospho T180) + MST1 (phospho T183) antibody
  • Description
    Rabbit polyclonal to STK3 (phospho T180) + MST1 (phospho T183)
  • Specificity
    ab79199 detects endogenous levels of STK3/MST1 only when phosphorylated at threonine 183 (MST1) and/or threonine T180 (STK3).
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide (phospho T183). Synthetic phosphopeptide derived from human STK3/MST1 around the phosphorylation site of threonine 183 (R-N-TP-V-I).

  • Positive control
    • WB: Extracts from 293 cells treated with H2O2 (100uM, 15mins). IHC-P: Human liver carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.
ELISA 1/40000.
IHC-P 1/50 - 1/100.

Target

Images

  • All lanes : Anti-STK3 (phospho T180) + MST1 (phospho T183) antibody (ab79199)

    Lane 1 : Extracts from 293 cells, treated with H2O2 (100uM, 15mins)
    Lane 2 : Extracts from 293 cells, treated with H2O2 (100uM, 15mins) with immunising phosphopeptide


    Predicted band size : 56 kDa
    Observed band size : 56 kDa
  • ab79199, at 1/50 dilution, staining STK3 (phospho T180) + MST1 (phospho T183) in human liver carcinoma by Immunohistochemistry using paraffin-embedded tissue, in the absence or presence of the immunising phosphopeptide.

References

This product has been referenced in:
  • Wu W  et al. Early protective role of MST1 knockdown in response to experimental diabetic nephropathy. Am J Transl Res 8:1397-411 (2016). Read more (PubMed: 27186267) »
  • Dixit D  et al. Chaetocin-induced ROS-mediated apoptosis involves ATM-YAP1 axis and JNK-dependent inhibition of glucose metabolism. Cell Death Dis 5:e1212 (2014). WB ; Human . Read more (PubMed: 24810048) »

See all 3 Publications for this product

Customer reviews and Q&As

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I have located an order for ab79199, but am not sure if this is the correct one. For ab63474, the PO or order number was not included in your emai...

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I am very sorry to hear that the tips did not help to improve the results.

Please let me know your order or PO number. If ab63474 was purchased within the last 6 months, I can replace, credit or refund the antib...

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Since ab79199 has not been tried on mouse samples, we do not guarantee it for this species. The guarantee applies for tested species and applications only.

I would like to suggest trying other antigen retr...

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I am sorry to hear that the antibodies are not working as expected.

Your protocol looks fine to me. I wanted to ask you a few more questions:

1) Both antibodies detect a phospho-site. Under the expe...

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