Anti-STK31 antibody (ab155172)
Key features and details
- Rabbit polyclonal to STK31
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-STK31 antibody
See all STK31 primary antibodies -
Description
Rabbit polyclonal to STK31 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
LCSLIYFRSSMTAEQVL
, corresponding to amino acids 952-968 of Mouse STK31 (NP_084192.2). -
Positive control
- Stk31 transfected AZ521 cells; ovarian carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 116 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) can also be used.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 116 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Tris-EDTA buffer (pH8.0) can also be used. |
Target
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Tissue specificity
Testis specific. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain.
Contains 1 Tudor domain. - Information by UniProt
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Database links
- Entrez Gene: 56164 Human
- Omim: 605790 Human
- SwissProt: Q9BXU1 Human
- Unigene: 309767 Human
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Alternative names
- Serine/threonine kinase 31 antibody
- Serine/threonine protein kinase 31 antibody
- Serine/threonine protein kinase NYD SPK antibody
see all
Images
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All lanes : Anti-STK31 antibody (ab155172) at 1/1000 dilution
Lane 1 : AZ521 whole cell lysate
Lane 2 : AZ521-STK31-GFP whole cell lysate
Lane 3 : AZ521-STK31-Myc-His whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 116 kDa
5% SDS PAGE -
Immunohistichemical analysis of paraffin-embedded human ovarian carcinoma tissue, labeling STK31 with ab155172 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab155172 has been referenced in 2 publications.
- Bae DH et al. STK31 upregulation is associated with chromatin remodeling in gastric cancer and induction of tumorigenicity in a xenograft mouse model. Oncol Rep 45:N/A (2021). PubMed: 33649810
- Nie Y et al. FANCD2 is required for the repression of germline transposable elements. Reproduction 159:659-668 (2020). PubMed: 32163912