Overview

  • Product nameAnti-STK31 antibody
    See all STK31 primary antibodies
  • Description
    Mouse monoclonal to STK31
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cynomolgus Monkey
  • Immunogen

    Recombinant fragment with a tag: WLSVQNQEFE INKDGIPKVD QFHLDDKVKS LLCSLICYRS SMTAEQVLNA ECFLMPKEQS VPNPEKDTEY TLYKKEEEIK TENLDKCMEK TRNGEANFDC, corresponding to amino acids 920-1019 of human STK31 (NP_113602). The tag sequence is not included here.

  • Positive control
    • STK31 transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab76839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 116 kDa.

Target

  • Tissue specificityTestis specific.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
    Contains 1 Tudor domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Serine/threonine kinase 31 antibody
    • Serine/threonine protein kinase 31 antibody
    • Serine/threonine protein kinase NYD SPK antibody
    • Serine/threonine-protein kinase 31 antibody
    • Serine/threonine-protein kinase NYD-SPK antibody
    • SgK396 antibody
    • STK 31 antibody
    • Stk31 antibody
    • STK31_HUMAN antibody
    • Sugen kinase 396 antibody
    • TDRD8 antibody
    • Tudor domain containing 8 antibody
    see all

Anti-STK31 antibody images

  • Anti-STK31 antibody (ab76839) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 116 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-STK31 antibody (ab76839) at 5 µg/ml

    Lane 1 : STK31 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 116 kDa
    Observed band size : 116 kDa
    Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

References for Anti-STK31 antibody (ab76839)

ab76839 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (ES cells)
Loading amount 20 µg
Specification ES cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted Dec 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"