Anti-STK31 antibody - N-terminal (ab71698)

Overview

  • Product name
    Anti-STK31 antibody - N-terminal
    See all STK31 primary antibodies
  • Description
    Rabbit polyclonal to STK31 - N-terminal
  • Tested applications
    Suitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide from the N terminal region of human STK31 conjugated to KLH.

  • Positive control
    • Rat testis tissue lysate. Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab71698 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 116 kDa).

Target

  • Tissue specificity
    Testis specific.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
    Contains 1 Tudor domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Serine/threonine kinase 31 antibody
    • Serine/threonine protein kinase 31 antibody
    • Serine/threonine protein kinase NYD SPK antibody
    • Serine/threonine-protein kinase 31 antibody
    • Serine/threonine-protein kinase NYD-SPK antibody
    • SgK396 antibody
    • STK 31 antibody
    • Stk31 antibody
    • STK31_HUMAN antibody
    • Sugen kinase 396 antibody
    • TDRD8 antibody
    • Testis tissue sperm-binding protein Li 78m antibody
    • Tudor domain containing 8 antibody
    see all

Images

  • Anti-STK31 antibody - N-terminal (ab71698) at 1/100 dilution + rat testis tissue lysate at 12.5 µg

    Predicted band size : 116 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,40 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ab71698, at 1/50 dilution, staining STK31 in human breast carcinoma by Immunohistochemistry using formalin fixed, paraffin embedded tissue. ab71698 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References

ab71698 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (ES cells)
Loading amount
20 µg
Specification
ES cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Verified customer

Submitted Dec 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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