Anti-STK31 antibody - N-terminal (ab71698)

Overview

  • Product nameAnti-STK31 antibody - N-terminal
    See all STK31 primary antibodies
  • Description
    Rabbit polyclonal to STK31 - N-terminal
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide from the N terminal region of human STK31 conjugated to KLH.

  • Positive control
    • Rat testis tissue lysate. Human breast carcinoma tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab71698 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab71698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 100 kDa (predicted molecular weight: 116 kDa).

Target

  • Tissue specificityTestis specific.
  • Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
    Contains 1 Tudor domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Serine/threonine kinase 31 antibody
    • Serine/threonine protein kinase 31 antibody
    • Serine/threonine protein kinase NYD SPK antibody
    • Serine/threonine-protein kinase 31 antibody
    • Serine/threonine-protein kinase NYD-SPK antibody
    • SgK396 antibody
    • STK 31 antibody
    • Stk31 antibody
    • STK31_HUMAN antibody
    • Sugen kinase 396 antibody
    • TDRD8 antibody
    • Tudor domain containing 8 antibody
    see all

Anti-STK31 antibody - N-terminal images

  • Anti-STK31 antibody - N-terminal (ab71698) at 1/100 dilution + rat testis tissue lysate at 12.5 µg

    Predicted band size : 116 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa,40 kDa,70 kDa. We are unsure as to the identity of these extra bands.
  • ab71698, at 1/50 dilution, staining STK31 in human breast carcinoma by Immunohistochemistry using formalin fixed, paraffin embedded tissue. ab71698 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

References for Anti-STK31 antibody - N-terminal (ab71698)

ab71698 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (ES cells)
Loading amount 20 µg
Specification ES cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
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Submitted Dec 20 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"