Overview

  • Product name
  • Description
    Rabbit polyclonal to STK33
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 55-469 of Human STK33 (Uniprot ID: Q9BYT3).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; HeLa cells; Human Cal27 xenograft tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab155056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Tissue specificity
    Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization
    Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • Hypothetical protein FLJ35932 antibody
    • Serine threonine kinase 33 antibody
    • Serine/threonine kinase 33 antibody
    • Serine/threonine protein kinase 33 antibody
    • Serine/threonine-protein kinase 33 antibody
    • STK 33 antibody
    • Stk33 antibody
    • STK33_HUMAN antibody
    see all

Images

  • Anti-STK33 antibody (ab155056) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS-PAGE.
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling STK33 with ab155056 at 1/200 dilution. Lower image is merged with DNA probe.
  • Immunohistochemical analysis of paraffin-embedded Human Cal27 xenograft tissue labeling STK33 with ab155056 at 1/100 dilution.

References

ab155056 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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