Overview

  • Product name
  • Description
    Goat polyclonal to STK35
  • Tested applications
    Suitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig
  • Immunogen

    Synthetic peptide:

    ELETRMDQVTCAA

    , corresponding to C terminal amino acids 389-401 of Human STK35

  • Positive control
    • Human Testis lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab26065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/16000.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
IHC-P Use a concentration of 10 µg/ml.

Target

  • Tissue specificity
    Expressed in testis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Nucleus. Nucleus > nucleolus. Cytoplasm. When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers (PubMed:11973348). However, PubMed:19756140 detected STK35 only in the nucleus, and the presence of PDLIM1 had no influence on its location.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLIK-1 antibody
    • Clik1 antibody
    • CLP 36 interacting kinase antibody
    • CLP-36-interacting kinase 1 antibody
    • EC 2.7.1.37 antibody
    • PDLIM1 interacting kinase antibody
    • PDLIM1-interacting kinase 1 antibody
    • Serine threonine kinase 35 long form antibody
    • Serine/threonine kinase 35 antibody
    • Serine/threonine protein kinase 35 antibody
    • Serine/threonine-protein kinase 35 antibody
    • Serine/threonine-protein kinase 35 L1 antibody
    • STK35 antibody
    • STK35_HUMAN antibody
    • STK35L1 antibody
    see all

Images

  • Anti-STK35 antibody (ab26065) at 1 µg/ml + Human Testis lysate (35µg protein in RIPA buffer).
    Developed using the ECL technique

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
  • ab26065 (10µg/ml) staining of Human Testis by IHC-P. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

References

This product has been referenced in:
  • Goyal P  et al. Identifying and characterizing a novel protein kinase STK35L1 and deciphering its orthologs and close-homologs in vertebrates. PLoS One 4:e6981 (2009). WB . Read more (PubMed: 19756140) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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