Overview

  • Product name
  • Description
    Rabbit polyclonal to STK35
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human STK35 aa 374-404 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Mouse kidney tissue lysate, Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab37974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Tissue specificity
    Expressed in testis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Nucleus. Nucleus > nucleolus. Cytoplasm. When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers (PubMed:11973348). However, PubMed:19756140 detected STK35 only in the nucleus, and the presence of PDLIM1 had no influence on its location.
  • Information by UniProt
  • Database links
  • Alternative names
    • CLIK-1 antibody
    • Clik1 antibody
    • CLP 36 interacting kinase antibody
    • CLP-36-interacting kinase 1 antibody
    • EC 2.7.1.37 antibody
    • PDLIM1 interacting kinase antibody
    • PDLIM1-interacting kinase 1 antibody
    • Serine threonine kinase 35 long form antibody
    • Serine/threonine kinase 35 antibody
    • Serine/threonine protein kinase 35 antibody
    • Serine/threonine-protein kinase 35 antibody
    • Serine/threonine-protein kinase 35 L1 antibody
    • STK35 antibody
    • STK35_HUMAN antibody
    • STK35L1 antibody
    see all

Images

  • Anti-STK35 antibody (ab37974) at 1/100 dilution + Mouse kidney tissue lysate

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.
  • ab37974, at a 1/50 dilution, staining STK35 in Human breast carcinoma by Immunohistochemistry, Formalin fixed Paraffin embedded tissue. ab37974 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

ab37974 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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