Overview

  • Product name
    Anti-STK35 antibody
    See all STK35 primary antibodies
  • Description
    Rabbit polyclonal to STK35
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human STK35 aa 374-404 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • Positive control
    • Mouse kidney tissue lysate, Human breast carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab37974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
ELISA 1/1000.
IHC-P 1/50 - 1/100.

Target

  • Tissue specificity
    Expressed in testis.
  • Sequence similarities
    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localization
    Nucleus. Nucleus > nucleolus. Cytoplasm. When associated with PDLIM1, it is mostly found in cytoplasm, localized to actin stress fibers (PubMed:11973348). However, PubMed:19756140 detected STK35 only in the nucleus, and the presence of PDLIM1 had no influence on its location.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • CLIK-1 antibody
    • Clik1 antibody
    • CLP 36 interacting kinase antibody
    • CLP-36-interacting kinase 1 antibody
    • EC 2.7.1.37 antibody
    • PDLIM1 interacting kinase antibody
    • PDLIM1-interacting kinase 1 antibody
    • Serine threonine kinase 35 long form antibody
    • Serine/threonine kinase 35 antibody
    • Serine/threonine protein kinase 35 antibody
    • Serine/threonine-protein kinase 35 antibody
    • Serine/threonine-protein kinase 35 L1 antibody
    • STK35 antibody
    • STK35_HUMAN antibody
    • STK35L1 antibody
    see all

Images

  • Western blot - Anti-STK35 antibody (ab37974)
    Western blot - Anti-STK35 antibody (ab37974)
    Anti-STK35 antibody (ab37974) at 1/100 dilution + Mouse kidney tissue lysate

    Predicted band size: 44 kDa
    Observed band size: 44 kDa
    Additional bands at: 15 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK35 antibody (ab37974)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-STK35 antibody (ab37974)
    ab37974, at a 1/50 dilution, staining STK35 in Human breast carcinoma by Immunohistochemistry, Formalin fixed Paraffin embedded tissue. ab37974 was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

References

ab37974 has not yet been referenced specifically in any publications.

Publishing research using ab37974? Please let us know so that we can cite the reference in this datasheet.

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