Overview

  • Product nameAnti-STK38 antibody
    See all STK38 primary antibodies
  • Description
    Mouse monoclonal to STK38
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 365-466 of Human STK38

Properties

Applications

Our Abpromise guarantee covers the use of ab56977 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 1 µg/ml.
Flow Cyt Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionNegative regulator of MAP3K1/2 signaling. Converts MAP3K2 from its phosphorylated form to its nonphosphorylated form and inhibits autophosphorylation of MAP3K2.
  • Tissue specificityUbiquitously expressed with highest levels observed in peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    ISGylated.
  • Cellular localizationNucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • NDR 1 antibody
    • NDR antibody
    • Ndr Ser/Thr kinase-like protein antibody
    • NDR1 antibody
    • NDR1 protein kinase antibody
    • Nuclear Dbf2 related kinase 1 antibody
    • nuclear Dbf2-related 1 antibody
    • Nuclear Dbf2-related kinase 1 antibody
    • Nuclear Dbf2-related protein antibody
    • Serine/threonine protein kinase 38 antibody
    • Serine/threonine-protein kinase 38 antibody
    • STK 38 antibody
    • STK38 antibody
    • STK38_HUMAN antibody
    see all

Anti-STK38 antibody images

  • STK38 antibody (ab56977) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human stomach.


  • Predicted band size : 54 kDa
    STK38 antibody (ab56977) at 1ug/lane + HeLa cell lysate at 25ug/lane.
  • Overlay histogram showing Jurkat cells stained with ab56977 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56977, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-STK38 antibody (ab56977)

ab56977 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"