STK38 antibody (ab56977) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human stomach.
Western blot - STK38 antibody (ab56977)
Predicted band size : 54 kDa STK38 antibody (ab56977) at 1ug/lane + HeLa cell lysate at 25ug/lane.
Flow Cytometry-Anti-STK38 antibody(ab56977)
Overlay histogram showing Jurkat cells stained with ab56977 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56977, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
References for Anti-STK38 antibody (ab56977)
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