Overview

  • Product nameAnti-STK39 antibody
    See all STK39 primary antibodies
  • Description
    Rabbit polyclonal to STK39
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide from the central region of human STK39 conjugated to KLH

  • Positive control
    • Mouse liver tissue lysate. Human hepatocarcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71825 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).

Target

  • FunctionMay act as a mediator of stress-activated signals.
  • Tissue specificityPredominantly expressed in brain and pancreas followed by heart, lung, kidney, skeletal muscle, liver, placenta and testis.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
    Contains 1 protein kinase domain.
  • DomainPAPA box (proline-alanine repeats) may target the kinase to a specific subcellular location by facilitating interaction with intracellular proteins such as actin or actin-like proteins.
  • Cellular localizationCytoplasm. Nucleus. Nucleus when caspase-cleaved.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCHT antibody
    • DKFZp686K05124 antibody
    • OTTHUMP00000165175 antibody
    • PASK antibody
    • Proline alanine rich STE20 related kinase antibody
    • Serine threonine kinase 39 (STE20/SPS1 homolog yeast) antibody
    • Serine threonine kinase 39 antibody
    • Serine/threonine protein kinase 39 antibody
    • Serine/threonine-protein kinase 39 antibody
    • Small intestine SPAK like kinase antibody
    • SPAK antibody
    • Ste 20 related kinase antibody
    • Ste-20-related kinase antibody
    • Ste20 like protein kinase antibody
    • STE20/SPS1 homolog antibody
    • STE20/SPS1 related proline alanine rich protein kinase antibody
    • STE20/SPS1-related proline-alanine-rich protein kinase antibody
    • STK 39 antibody
    • Stk39 antibody
    • STK39_HUMAN antibody
    see all

Anti-STK39 antibody images

  • Anti-STK39 antibody (ab71825) at 1/100 dilution + mouse liver tissue lysate at 12.5 µg

    Secondary
    HRP-anti-rabbit

    Predicted band size : 60 kDa
    Observed band size : 60 kDa
    Additional bands at : 10 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • ab71825 at 1/50 dilution, staining STK39 in human hepatocarcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and DAB staining.
  • ICC/IF image of ab71825 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71825, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-STK39 antibody (ab71825)

This product has been referenced in:
  • Pandiri AR  et al. Differential transcriptomic analysis of spontaneous lung tumors in B6C3F1 mice: comparison to human non-small cell lung cancer. Toxicol Pathol 40:1141-59 (2012). IHC-P ; Mouse . Read more (PubMed: 22688403) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab71825.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"