The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 21 kDa.
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
FunctionIs a key regulator of neurite extension through regulation of microtubule instabilily.
Tissue specificityNeuron specific.
Sequence similaritiesBelongs to the stathmin family.
Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Cell projection > growth cone. Membrane. Cell projection > axon. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. SCG10 exists in both soluble and membrane-bound forms.
ab115513 at 5ug/ml staining STMN2 in Formalin-fixed, Paraffin-embedded Human brain (cortex) tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
ab115513 at 5ug/ml staining STMN2 in Formalin-fixed, Paraffin-embedded Human small intestine (myenteric plexus) tissue Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Western blot - Anti-STMN2 antibody (ab115513)
Anti-STMN2 antibody (ab115513) at 1 µg/ml + Human brain lysate (in RIPA buffer) at 35 µg developed using the ECL technique
Predicted band size : 21 kDa
References for Anti-STMN2 antibody (ab115513)
has not yet been referenced specifically in any publications.
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