Overview

  • Product name
  • Description
    Goat polyclonal to STMN2
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide:

    AKTAMAYKEK

    , corresponding to N terminal amino acids 2-11 of STMN2

  • Positive control
    • Human Brain lysate, A431 lysate ICC/IF: HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab21190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.

This product gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min).

WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 20.8 kDa.

Target

  • Function
    Is a key regulator of neurite extension through regulation of microtubule instabilily.
  • Tissue specificity
    Neuron specific.
  • Sequence similarities
    Belongs to the stathmin family.
  • Post-translational
    modifications
    Sumoylated.
  • Cellular localization
    Cytoplasm. Cytoplasm > perinuclear region. Cell projection > growth cone. Membrane. Cell projection > axon. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. SCG10 exists in both soluble and membrane-bound forms.
  • Information by UniProt
  • Database links
  • Alternative names
    • Neuron specific growth associated protein antibody
    • Neuronal growth associated protein antibody
    • Neuronal growth associated protein (silencer element) antibody
    • Protein SCG10 antibody
    • SCG 10 antibody
    • SCG10 antibody
    • SCG10 protein antibody
    • SCGN 10 antibody
    • SCGN10 antibody
    • SGC 10 antibody
    • SGC10 antibody
    • Stathmin 2 antibody
    • Stathmin like 2 antibody
    • Stathmin-2 antibody
    • STMN 2 antibody
    • STMN2 antibody
    • STMN2_HUMAN antibody
    • Superior cervical ganglia neural specific 10 antibody
    • Superior cervical ganglion 10 protein antibody
    • Superior cervical ganglion-10 protein antibody
    • Superiorcervical ganglia neural specific 10 antibody
    see all

Images

  • Ab211990 staining TAF9 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab211990 at 1/100 dilution (pseudocolored in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Anti-STMN2 antibody (ab21190) at 1 µg/ml + Human Brain lysate (RIPA buffer, 35µg total protein per lane).

    Predicted band size : 20.8 kDa
    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References

This product has been referenced in:
  • Davis EJ  et al. Intrastriatal dopamine D1 antagonism dampens neural plasticity in response to motor cortex lesion. Neuroscience 146:784-91 (2007). Read more (PubMed: 17331653) »
  • Ohkawa N  et al. The microtubule destabilizer stathmin mediates the development of dendritic arbors in neuronal cells. J Cell Sci 120:1447-56 (2007). Read more (PubMed: 17389683) »

See all 2 Publications for this product

Customer reviews and Q&As

I'm sorry to hear you are having a problem with ab21190. This antibody has been tested in human brain lysate lysed in RIPA buffer containing protease inhibitors. You do not mention which type of cell lysate you have used and it may be that your sam...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I am most surprised by the results that your customer has been obtaining. We recommend that the antibody can be applied in a dilution range of ...

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Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab21190. Please note that this antibody is a "Fast-track" antibody and has not yet been fully characterized. Preliminary Western blot experiments were done using...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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